Origin and structure of the group-specific, complement-fixing antigen of Rickettsia rickettsii.

Applied microbiology ·Vol. 28 ·No. 3 ·1974-09-00 ·Pages 481-8

Tzianabos T, Palmer EL, Obijeski JF, Martin ML

Abstract

Rickettsia rickettsii was treated with ether and examined by negative-contrast electron microscopy. Group-specific complement-fixing antigen was seen to be originating from the cell wall. The antigen was composed predominately of round particles 10 to 60 nm in diameter. Intact R. rickettsii and antigen from ether-treated organisms were purified by density gradient centrifugation and analyzed by polyacrylamide gel electrophoresis. The whole rickettsial cell was composed of a minimum of 30 proteins which ranged in molecular weight from about 23,000 to 155,000. The "soluble" antigen contained nine proteins ranging in molecular weight from about 28,000 to 150,000.

MeSH Terms

Antigens, Bacterial/analysis,isolation & purification Bacterial Proteins/analysis Cell Wall/immunology,ultrastructure Centrifugation, Density Gradient Complement Fixation Tests Ethyl Ethers/pharmacology Microscopy, Electron Molecular Weight Rickettsia rickettsii/analysis,immunology,ultrastructure

Chemicals

Antigens, Bacterial Bacterial Proteins Ethyl Ethers

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Tzianabos T

Palmer E L

Obijeski J F

Martin M L

References (13)

13 references, click to expand

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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.28.3.481-488.1974

Published

1974-09-00

Pages

481-8

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC186747

Subset

External Links

PubMed source DOI: 10.1128/am.28.3.481-488.1974 PMC Full Text

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Origin and structure of the group-specific, complement-fixing antigen of Rickettsia rickettsii.

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