Aldosterone-induced protein in toad urinary bladder.

Science (New York, N.Y.) ·Vol. 186 ·No. 4160 ·1974-10-18 ·Pages 269-72

Benjamin WB, Singer I

Abstract

Simultaneous electrophysiological and biochemical experiments demonstrated a specific aldosterone-induced protein in paired urinary hemibladders isolated from the toad Bufo marinus. Whenever aldosterone stinlmulated short-circuit current, aldosterone specifically increased [(35)S] methionine incorporation into a low-molecular-weight protein (about 12,000). Comparative studies with dexamethasone and insulin and inhibitory studies with spironolactone and actinomycin D suggest mineralocorticoid specificity.

MeSH Terms

Aldosterone/pharmacology Animals Biological Transport, Active Bufo marinus Dactinomycin/pharmacology In Vitro Techniques Insulin/pharmacology Kinetics Membrane Potentials Methionine/metabolism Mineralocorticoid Receptor Antagonists Molecular Weight Peptide Biosynthesis Protein Biosynthesis Sodium/metabolism Spironolactone/pharmacology Stimulation, Chemical Sulfur Radioisotopes Urinary Bladder/metabolism,physiology

Chemicals

Insulin Mineralocorticoid Receptor Antagonists Sulfur Radioisotopes Dactinomycin Spironolactone Aldosterone Sodium Methionine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Benjamin W B

Singer I

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.186.4160.269

Published

1974-10-18

Pages

269-72

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.186.4160.269 View journal details

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Aldosterone-induced protein in toad urinary bladder.

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