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PMID: 4208551 Published · ppublish English Journal Article

Partial purification of detergent-soluble HL-A antigen and its cleavage by papain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 4 ·1974-04-00 ·Pages 1539-43

Springer TA, Strominger JL, Mann D

Abstract

HL-A antigen solubilized with the non-ionic detergent, Brij 99, has been purified to about 50% of homogeneity from a cultured human lymphoblast line. It consists of two nonidentical subunits of 44,000 and 12,000 molecular weight (MW). Upon papain proteolysis the 44,000 MW peptide is converted by at least two cleavages to a 34,000 MW peptide, but the 12,000 MW peptide appears to be unchanged. Concomitantly, the apparent molecular weight in gel filtration chromatography under nondenaturing conditions in the presence of Brij 99 is reduced from 460,000 to 45,000. HL-A molecules produced by direct papain proteolysis of membranes and by papain treatment of purified detergent-soluble HL-A are identical.

MeSH Terms
Animals Cell Line Chromatography, Affinity Chromatography, DEAE-Cellulose Chromatography, Gel Chromium Radioisotopes Detergents Edetic Acid Histocompatibility Antigens/isolation & purification Humans Hydrolysis Lymphocytes/immunology Mice Molecular Weight Papain Peptide Fragments/analysis Precipitin Tests Serum Albumin, Bovine Sodium Dodecyl Sulfate
Chemicals
Chromium Radioisotopes Detergents Histocompatibility Antigens Peptide Fragments Serum Albumin, Bovine Sodium Dodecyl Sulfate Edetic Acid Papain
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Springer T A
Strominger J L
Mann D
References (35)
35 references, click to expand
  1. The small subunit of HL-A antigens is beta 2-microglobulin.
    J Exp Med. 1973 Dec 1;138(6):1608-12 PMID: 4128442
  2. Purification and characterization of HL-A antigens from human platelets, solubilized by the non-ionic detergent NP-40.
    Biochim Biophys Acta. 1973 Apr 16;298(4):783-9 PMID: 4729817
  3. The purification of detergent-solubilized HL-A antigens by affinity chromatography with the hemagglutinin from Lens culinaris.
    J Immunol. 1974 Mar;112(3):1190-3 PMID: 4811965
  4. Biochemical properties of histocompatibility antigens.
    Annu Rev Genet. 1970;4(0):69-90 PMID: 4136564
  5. Isolation of H-2 alloantigens solubilized by the detergent NP-40.
    J Immunol. 1971 Nov;107(5):1363-7 PMID: 5171127
  6. Peptides obtained by tryptic hydrolysis of performic acid-oxidized ribonuclease.
    J Biol Chem. 1956 Apr;219(2):623-42 PMID: 13319284
  7. Three closely linked genetic systems relevant to transplantation.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3031-5 PMID: 4399834
  8. Lymphocyte activation and destruction in vitro in relation to MLC and HL-A.
    Transplant Proc. 1973 Mar;5(1):415-20 PMID: 4121172
  9. Characterization of soluble substances in plasma carrying HL-A alloantigenic activity and HL-A common antigenic activity.
    Transplantation. 1973 Mar;15(3):312-9 PMID: 4123078
  10. Studies on phytohemagglutinins. IV. Isolation and characterization of a hemagglutinin from the lentil, Lens, esculenta, Moench.
    Biochim Biophys Acta. 1970 Nov 17;221(2):282-9 PMID: 5490235
  11. Multiple common properties of human beta2-microglobulin and the common portion fragment derived from HL-A antigen molecules.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2863-5 PMID: 4517941
  12. Human lymphocyte membrane (HL-A) alloantigens: isolation, purification and properties.
    J Immunol. 1969 Aug;103(2):282-92 PMID: 5803787
  13. Fc and Fab fragments from IgG 2 human immunoglobulins characterized.
    Nat New Biol. 1972 Nov 1;240(96):24-6 PMID: 4508296
  14. HL-A substances from human spleens.
    Nature. 1968 Oct 12;220(5163):192-5 PMID: 5684840
  15. Bile acid content of human serum. II. The binding of cholanic acids by human plasma proteins.
    J Clin Invest. 1957 Apr;36(4):538-42 PMID: 13416382
  16. Molecular heterogeneity of human lymphoid (HL-A) alloantigens.
    Science. 1969 Mar 28;163(3874):1460-2 PMID: 5773111
  17. Stepwise cleavage of rabbit immunoglobulin G by papain and isolation of four types of biologically active Fc fragments.
    Biochem J. 1969 Apr;112(3):343-55 PMID: 4979349
  18. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  19. H-2 histocompatibility alloantigens. Some biochemical properties of the molecules solubilized by NP-40 detergent.
    Biochemistry. 1973 May 22;12(11):2157-64 PMID: 4196220
  20. QUANTITATIVE TITRATIONS OF MOUSE H-2 ANTIBODIES USING CR-51-LABELLED TARGET CELLS.
    Transplantation. 1965 May;3:423-31 PMID: 14285093
  21. Salt extraction of soluble HL-A antigens.
    Science. 1971 Jun 11;172(3988):1134-6 PMID: 5574518
  22. Ragweed hay fever: genetic control and linkage to HL-A haplotypes.
    Science. 1972 Dec 15;178(4066):1201-3 PMID: 4637806
  23. Polypeptides of the tail fibres of bacteriophage T4.
    J Mol Biol. 1971 Dec 28;62(3):465-77 PMID: 5136579
  24. Subcellular separation and molecular nature of human histocompatibility antigens (HL-A).
    Nature. 1974 Feb 15;247(5441):457-61 PMID: 4131934
  25. CYTOTOXIC REACTIONS OF MOUSE ISO-ANTISERA: PRELIMINARY CONSIDERATIONS.
    Br J Exp Pathol. 1964 Aug;45:398-408 PMID: 14203195
  26. The effect of enzyme inhibitors on the solubilization of HL-A antigens with 3 M KCl.
    Transplantation. 1972 Sep;14(3):398-401 PMID: 4637813
  27. Isolation of glycoproteins from pig lymphocyte plasma membrane using Lens culinaris phytohemagglutinin.
    Biochem Biophys Res Commun. 1972 May 26;47(4):923-30 PMID: 5026302
  28. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.
    Biochem J. 1963 Oct;89:114-23 PMID: 14097352
  29. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  30. Fractionation of murine H-2 antigens with the use of detergents.
    Transplantation. 1969 Oct;8(4):451-61 PMID: 5310056
  31. Murine histocompatibility-2 (H-2) alloantigens. Purification and some chemical properties of soluble products from H-2b and H-2d genotypes released by papain digestion of membrane fractions.
    Biochemistry. 1969 Oct;8(10):4048-62 PMID: 5346386
  32. Highly purified papain-solubilized HL-A antigens contain beta2-microglobulin.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):35-9 PMID: 4129801
  33. Papain-solubilized HL-A antigens from cultured human lymphocytes contain two peptide fragments.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1603-7 PMID: 4514327
  34. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid.
    J Biol Chem. 1960 Mar;235:769-75 PMID: 13793161
  35. Histocompatibility-linked immune response genes.
    Science. 1972 Jan 21;175(4019):273-9 PMID: 4109878
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.4.1539
Published
1974-04-00
Pages
1539-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388266
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.4.1539 PMC Full Text View journal details
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