Use of ultraviolet radiation to locate dipicolinic acid in Bacillus cereus spores.

Journal of bacteriology ·Vol. 118 ·No. 1 ·1974-04-00 ·Pages 202-8

Germaine GR, Murrell WG

Abstract

A novel method is described that allows a direct determination of the location of a portion of the dipicolinic acid (DPA) in spores. Ultraviolet (UV) irradiation is shown to cause cross-linkage of DPA to spore proteins which have the characteristics of membrane proteins. We suggest that DPA resides in the inner forespore membrane (IFSM) and spore cytoplasm (i.e., the spore protoplast). Only that portion of the DPA in the vicinity of the IFSM appeared to form UV-induced DPA-protein adducts.

MeSH Terms

Bacillus cereus/analysis,metabolism,radiation effects Bacterial Proteins/metabolism Carbon Radioisotopes Cell Fractionation Cell Membrane/analysis Centrifugation, Density Gradient Cytoplasm/analysis Electrophoresis, Polyacrylamide Gel Methods Picolinic Acids/isolation & purification,metabolism Protein Binding/radiation effects Radiation Effects Spores, Bacterial/analysis,metabolism,radiation effects Tritium Ultraviolet Rays

Chemicals

Bacterial Proteins Carbon Radioisotopes Picolinic Acids Tritium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Germaine G R

Murrell W G

References (18)

18 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.118.1.202-208.1974

Published

1974-04-00

Pages

202-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC246658

Subset

External Links

PubMed source DOI: 10.1128/jb.118.1.202-208.1974 PMC Full Text View journal details

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Use of ultraviolet radiation to locate dipicolinic acid in Bacillus cereus spores.

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