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PMID: 4202992 Published · ppublish English Journal Article

Absence of 3'-terminal residues from transfer ribonucleic acid of dormant spores of Bacillus megaterium.

Journal of bacteriology ·Vol. 117 ·No. 1 ·1974-01-00 ·Pages 126-32

Setlow P, Primus G, Deutscher MP

Abstract

Essentially all (>97%) of the transfer ribonucleic acid (tRNA) in log-phase and sporulating cells of Bacillus megaterium contains a complete 3'-cytidyl-cytidyl-adenosine terminus. However, about one-third of the tRNA in the dormant spore lacks the 3'-terminal adenosine 5'-monophosphate (AMP) residue, and some of the adjacent cytosine monophosphate residues are also missing. Examination of specific tRNAs indicated that those specific for isoleucine, leucine, and methionine are missing 30 to 40% of their terminal residue, whereas tRNAs specific for tyrosine lack 88% of the 3'-terminal AMP. Defective spore tRNA is not degraded during germination, but the missing residues are added back in the first minutes of the process. The enzyme catalyzing the addition reaction, tRNA nucleotidyltransferase, is present in the dormant spore at a level similar to that found in the vegetative cell.

MeSH Terms
Adenosine Monophosphate/analysis,metabolism Bacillus megaterium/analysis,enzymology,growth & development Base Sequence Carbon Radioisotopes Centrifugation, Density Gradient Chromatography, DEAE-Cellulose Cyanides/pharmacology Cytosine Nucleotides/analysis,metabolism Fluorides/pharmacology RNA Nucleotidyltransferases/metabolism RNA, Bacterial/analysis,isolation & purification RNA, Transfer/analysis,isolation & purification Spores, Bacterial/analysis,enzymology,growth & development
Chemicals
Carbon Radioisotopes Cyanides Cytosine Nucleotides RNA, Bacterial Adenosine Monophosphate RNA, Transfer RNA Nucleotidyltransferases Fluorides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Setlow P
Primus G
Deutscher M P
References (24)
24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.117.1.126-132.1974
Published
1974-01-00
Pages
126-32
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246533
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.117.1.126-132.1974 PMC Full Text View journal details
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