Genetic studies of leucine biosynthesis in Bacillus subtilis.

Journal of bacteriology ·Vol. 116 ·No. 2 ·1973-11-00 ·Pages 719-26

Ward JB, Zahler SA

Abstract

The mutations in a series of leucine auxotrophs isolated after treatment with nitrosoguanidine, ultraviolet light, and ICR-191 have been mapped between ilvC and pheA on the Bacillus subtilis chromosome. A fine structure map of the region was constructed by transformation. Analysis of several strains by assaying levels of their leucine bioysnthetic enzymes has shown that the region encodes three enzymes. The order of the genes with respect to the biosynthetic steps catalyzed by the gene products is 1-3-2.

MeSH Terms

Bacillus subtilis/drug effects,metabolism Bacterial Proteins/metabolism Chromosome Mapping Chromosomes, Bacterial Culture Media Genes Genetics, Microbial Genotype Leucine/biosynthesis Molecular Biology Mutation Penicillins/pharmacology Phenotype Transduction, Genetic Transformation, Genetic

Chemicals

Bacterial Proteins Culture Media Penicillins Leucine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ward J B

Zahler S A

References (11)

11 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.116.2.719-726.1973

Published

1973-11-00

Pages

719-26

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285437

Subset

External Links

PubMed source DOI: 10.1128/jb.116.2.719-726.1973 PMC Full Text View journal details

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