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PMID: 4200253 Published · ppublish English Journal Article

Studies of lipid A fractions from the lipopolysaccharides of Pseudomonas aeruginosa and Pseudomonas alcaligenes.

The Biochemical journal ·Vol. 133 ·No. 3 ·1973-07-00 ·Pages 563-72

Drewry DT, Lomax JA, Gray GW, Wilkinson SG

Abstract

Lipid A fractions from Pseudomonas aeruginosa and Pseudomonas alcaligenes have similar compositions and structural features. By means of hydrazinolysis of the parent lipopolysaccharides and partial hydrolysis of the deacylation products, it was established that both lipids are derived from the beta-(1-->6)-linked disaccharide of glucosamine. Phosphorylated derivatives of the disaccharide from Ps. aeruginosa were also characterized. The lipids differ mainly in the absence of hexadecanoic acid and 2-hydroxydodecanoic acid from the lipid from Ps. alcaligenes. Evidence that in Ps. aeruginosa these acids are ester-linked to residues of 3-hydroxyalkanoic acids (including 3-hydroxydecanoic acid) was obtained. Heterogeneity of lipid A fractions was indicated by t.l.c., and by gel filtration of de-O-acylation products from mild alkaline methanolysis of the lipids.

MeSH Terms
Acylation Alkaline Phosphatase/metabolism Alkanes/analysis Chromatography, Gas Chromatography, Gel Chromatography, Paper Chromatography, Thin Layer Disaccharides Electrophoresis, Paper Fatty Acids/analysis Glucosamine Hexosaminidases/metabolism Lipids/analysis,isolation & purification Lipopolysaccharides/isolation & purification Pseudomonas/analysis Pseudomonas aeruginosa/analysis
Chemicals
Alkanes Disaccharides Fatty Acids Lipids Lipopolysaccharides Alkaline Phosphatase Hexosaminidases Glucosamine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Drewry D T
Lomax J A
Gray G W
Wilkinson S G
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28 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1330563
Published
1973-07-00
Pages
563-72
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1177735
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1330563 PMC Full Text View journal details
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