The chain length of the glycans in bacterial cell walls.

The Biochemical journal ·Vol. 133 ·No. 2 ·1973-06-00 ·Pages 395-8

Ward JB

Abstract

The glycan chain length of peptidoglycan was measured by reduction with NaB(3)H(4) and isolation of the resulting muramitol, indicative of the length of the chains as biosynthesized, and glucosaminol, which measured the length of the chains after rupture by endo-beta-N-acetylglucosaminidases. Measurement of the non-reducing terminal N-acetylglucosamine by Smith degradation confirmed the result.

MeSH Terms

Acetamides Bacillus/analysis,cytology Bacillus subtilis/analysis,cytology Borohydrides Cell Wall/analysis Chromatography, Paper Electrophoresis, Paper Glucosamine/analysis Glycerol/analysis Methods Molecular Weight Monosaccharides/analysis Muramic Acids/analysis Polysaccharides, Bacterial/analysis Staphylococcus/analysis,cytology Sugar Alcohols Tritium

Chemicals

Acetamides Borohydrides Monosaccharides Muramic Acids Polysaccharides, Bacterial Sugar Alcohols Tritium Glucosamine Glycerol

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Ward J B

References (18)

18 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1330395

Published

1973-06-00

Pages

395-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177711

Subset

External Links

PubMed source DOI: 10.1042/bj1330395 PMC Full Text View journal details

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The chain length of the glycans in bacterial cell walls.

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