Selective extraction of polymers from cell walls of gram-positive bacteria.

The Biochemical journal ·Vol. 131 ·No. 3 ·1973-03-00 ·Pages 619-21

Pavlik JG, Rogers HJ

Abstract

Brief heating of Bacillus Licheniformis cell walls at 100 degrees C in aqueous buffers of pH3.0-4.0 removes some polymers but not others from the mucopeptides. For example, relatively undegraded teichuronic acid can be extracted at 100 degrees C in 20min at pH3.0 whereas the teichoic acids are not removed. Similar specificity can be shown with walls from three other species of micro-organism.

MeSH Terms

Bacillus/analysis,cytology Bacillus subtilis/analysis Bacteria/analysis Cell Wall/analysis Glycopeptides/analysis Hot Temperature Hydrogen-Ion Concentration Micrococcus/analysis Polysaccharides, Bacterial/isolation & purification Solubility Species Specificity Streptococcus/analysis Teichoic Acids/isolation & purification Time Factors Uronic Acids/analysis

Chemicals

Glycopeptides Polysaccharides, Bacterial Teichoic Acids Uronic Acids

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Pavlik J G

Rogers H J

References (14)

14 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1310619

Published

1973-03-00

Pages

619-21

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177511

Subset

External Links

PubMed source DOI: 10.1042/bj1310619 PMC Full Text View journal details

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Selective extraction of polymers from cell walls of gram-positive bacteria.

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