Kinetics of the diamine oxidase reaction.

The Biochemical journal ·Vol. 131 ·No. 3 ·1973-03-00 ·Pages 459-69

Bardsley WG, Crabbe MJ, Shindler JS

Abstract

1. The oxidation of p-dimethylaminomethylbenzylamine was followed spectrophotometrically by measuring the change in E(250) caused by the p-dimethylaminomethylbenzaldehyde produced under a wide variety of experimental conditions. 2. The effect of variations in concentrations of both substrates (amine and oxygen) and all products (aminoaldehyde, hydrogen peroxide and ammonia) on this reaction was studied and the results used to develop a formal mechanism. 3. The nature of the rate-limiting step was elucidated by studying the effects of alterations in ionic strength, dielectric constant and deuterium substitution on the velocity of the forward reaction. 4. Thermodynamic activation energy parameters were obtained at several pH values from the effects of temperature on the reaction.

MeSH Terms

Amine Oxidase (Copper-Containing)/antagonists & inhibitors Ammonia Animals Benzaldehydes Binding, Competitive Deuterium Dimethylamines Electric Conductivity Enzyme Activation Feedback Hydrogen Peroxide Kidney/enzymology Kinetics Mathematics Models, Chemical Osmolar Concentration Oxidation-Reduction Oxygen Partial Pressure Spectrophotometry, Ultraviolet Swine Temperature Thermodynamics Xylenes

Chemicals

Benzaldehydes Dimethylamines Xylenes 4-dimethylaminomethylbenzaldehyde Ammonia Deuterium Hydrogen Peroxide Amine Oxidase (Copper-Containing) Oxygen

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bardsley W G

Crabbe M J

Shindler J S

References (20)

20 references, click to expand

The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations.
Biochim Biophys Acta. 1963 Jan 8;67:104-37 PMID: 14021667
Mechanistic studies of beef plasma amine oxidase.
Biochemistry. 1970 Aug 18;9(17):3378-83 PMID: 5522469
Some aspects of catalysis by the amine oxidase of pea seedlings.
Biochemistry. 1970 Mar 3;9(5):1206-10 PMID: 5418714
Characterization of sodium borohydride-reduced histaminase-histamine intermediate.
Biochim Biophys Acta. 1969 Jul 8;185(1):242-4 PMID: 4978707
The effect of pH on the kinetic parameters and mechanism of beef liver monoamine oxidase.
Arch Biochem Biophys. 1971 Aug;145(2):557-64 PMID: 5125204
Synthesis and oxidation of aminoalkyl-onium compounds by pig kidney diamine oxidase.
Biochem J. 1971 May;122(4):557-67 PMID: 5001323
The reaction pathway of pig brain mitochondrial monoamine oxidase.
Eur J Biochem. 1968 Aug;5(3):316-20 PMID: 5680351
Evidence for a ping-pong mechanism in the diamine oxidase reaction.
FEBS Lett. 1969 Jul;4(1):31-32 PMID: 11947138
A reinvestigation of the substrate specificity of pig kidney diamine oxidase.
Biochem J. 1970 Mar;117(1):169-76 PMID: 4986966
Diamine oxidase inactivation by hydrogen peroxide.
Biochim Biophys Acta. 1967 Mar 15;132(2):521-3 PMID: 4961900
Indirect evidence for the production of superoxide anion radicals by pig kidney diamine oxidase.
Biochim Biophys Acta. 1970 Mar 18;198(3):618-20 PMID: 4985264
The kinetics of enzyme-catalyzed reactions with two or more substrates or products. III. Prediction of initial velocity and inhibition patterns by inspection.
Biochim Biophys Acta. 1963 Feb 12;67:188-96 PMID: 14021669
Diamine oxidase from pig kidney. Improved purification and properties.
J Biol Chem. 1967 Mar 25;242(6):1160-7 PMID: 4290315
Oxidation of p-dimethylaminomethylbenzylamine by pig kidney diamine oxidase. A new method for spectrophotometric assay.
Biochem J. 1972 May;127(5):875-9 PMID: 4627688
The enzymic degradation of alkyl-substituted gentisates, maleates and malates.
Biochem J. 1971 Mar;122(1):29-40 PMID: 5124802
The kinetics of enzyme-catalyzed reactions with two or more substrates or products. II. Inhibition: nomenclature and theory.
Biochim Biophys Acta. 1963 Feb 12;67:173-87 PMID: 14021668
Deuterium isotope effects in relation to the chemical mechanism of monoamine oxidase.
Ann N Y Acad Sci. 1963 Jul 8;107:822-39 PMID: 13970474
Copper reduction by substrate in diamine oxidase.
FEBS Lett. 1969 Jan;2(3):182-184 PMID: 11946306
Pyridoxal phosphate as a prosthetic group of pig kidney diamine oxidase.
Arch Biochem Biophys. 1967 Mar;119(1):373-81 PMID: 4964016
Inhibition of pig kidney diamine oxidase by substrate analogues.
Biochem J. 1972 Jun;128(2):253-63 PMID: 4628527

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1310459

Published

1973-03-00

Pages

459-69

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177494

Subset

External Links

PubMed source DOI: 10.1042/bj1310459 PMC Full Text View journal details

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