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PMID: 4195054 Published · ppublish English Journal Article

Morphology and entry of enveloped and deenveloped equine abortion (herpes) virus.

Journal of virology ·Vol. 5 ·No. 4 ·1970-04-00 ·Pages 513-23

Abodeely RA, Lawson LA, Randall CC

Abstract

Selective removal of the envelope of equine abortion (herpes) virus was accomplished by utilizing the nonionic detergent Nonidet P-40 followed by sonic treatment. The deenveloped particles differ significantly in size and buoyant density from the enveloped form. The cellular entry of purified enveloped and purified deenveloped virus was examined by electron microscopy during critical time periods. Both forms appeared to enter cells by a viropexis mechanism in which particles were engulfed by pseudopodia which either surround the virus and fuse with the cell membrane or to other pseudopodia, forming fusion vacuoles containing from one to numerous viral particles. This mode of entry was noted extensively at 5 min postinoculation. Deenveloped particles were apparently infectious only for hamsters, with a large inoculum being required. Contamination by enveloped forms was not noted after exhaustive search by electron microscopy.

MeSH Terms
Animals Cell Line Centrifugation, Density Gradient Connective Tissue Cricetinae Deoxyribonucleases/metabolism Detergents Herpesviridae/growth & development,isolation & purification,pathogenicity Herpesviridae Infections/blood Mice Microscopy, Electron Staining and Labeling Thymidine/metabolism Tritium Ultrasonics Vibration Viral Proteins/isolation & purification
Chemicals
Detergents Viral Proteins Tritium Deoxyribonucleases Thymidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Abodeely R A
Lawson L A
Randall C C
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32 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.5.4.513-523.1970
Published
1970-04-00
Pages
513-23
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC376034
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.5.4.513-523.1970 PMC Full Text View journal details
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