Nature of the ethylenediaminetetraacetic acid requirement for transformation of Bacillus subtilis with single-stranded deoxyribonucleic acid.

Journal of bacteriology ·Vol. 101 ·No. 3 ·1970-03-00 ·Pages 844-50

Tevethia MJ, Mandel M

Abstract

The ethylenediaminetetraacetate (EDTA) requirement for transformation of Bacillus subtilis with single-stranded deoxyribonucleic acid (DNA) was examined. The results indicate that a chelating agent such as EDTA is a stringent requirement for transformation with single DNA strands only at nonsaturating DNA concentrations, and that EDTA, when required, must be present during several steps in the transformation process and appears to insure the survival of single-stranded DNA by rendering a nuclease in competent populations inactive.

MeSH Terms

Bacillus subtilis/growth & development DNA, Bacterial/isolation & purification Deoxyribonucleases/metabolism Edetic Acid/pharmacology Protein Denaturation Staining and Labeling Transformation, Genetic/drug effects

Chemicals

DNA, Bacterial Edetic Acid Deoxyribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Tevethia M J

Mandel M

References (11)

11 references, click to expand

A NONSPECIFIC INCREASE IN PERMEABILITY IN ESCHERICHIA COLI PRODUCED BY EDTA.
Proc Natl Acad Sci U S A. 1965 Apr;53:745-50 PMID: 14324530
REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
Origin of the residual transforming activity of denatured Bacillus subtilis DNA.
J Mol Biol. 1968 Mar 14;32(2):369-77 PMID: 4966869
Cellular site in Bacillus subtilis of a nuclease which preferentially degrades single-stranded nucleic acids.
J Bacteriol. 1966 Mar;91(3):1004-11 PMID: 4956329
Residual transforming activity of denatured Haemophilus influenzae DNA.
J Mol Biol. 1968 Mar 14;32(2):437-51 PMID: 5300446
Uptake of "single-stranded" DNA in Hemophilus influenzae and its ability to transform.
J Mol Biol. 1966 Apr;16(2):317-27 PMID: 5297183
Further studies on transformation with single-stranded DNA of Hemophilus influenzae.
J Mol Biol. 1967 Sep 14;28(2):247-59 PMID: 4964030
Mechanism of the deoxyribonucleic acid helping effect during transformation.
J Mol Biol. 1968 May 14;33(3):873-91 PMID: 4387337
Efficient separation of single-stranded and double-stranded deoxyribonucleic acid in a dextran-polyethylene glycol two-phase system.
Biochemistry. 1967 Aug;6(8):2527-32 PMID: 4964524
Characterization of a naturally occurring, cross-linked fraction of DNA. 1. Nature of the cross-linkage.
J Mol Biol. 1968 Mar 14;32(2):379-403 PMID: 4966870
Residual activity of denatured transforming DNA of Haemophilus influenzae: a natrually occurring cross-linked DNA.
J Mol Biol. 1968 Mar 14;32(2):423-35 PMID: 5300445

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.101.3.844-850.1970

Published

1970-03-00

Pages

844-50

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC250400

Subset

External Links

PubMed source DOI: 10.1128/jb.101.3.844-850.1970 PMC Full Text View journal details

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Nature of the ethylenediaminetetraacetic acid requirement for transformation of Bacillus subtilis with single-stranded deoxyribonucleic acid.

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