Surface localization of Escherichia coli 5'-nucleotidase by electron microscopy.
The 5'-nucleotidase of Escherichia coli was shown to be located at the cell wall surface by histochemical techniques utilizing the deposition of inorganic phosphate. Penetration of the 5'-nucleotidase in the periplasmic space was seen only in cells treated with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)aminomethane (Tris). The 3'-nucleotidase of E. coli was also found to have a surface location, and periplasmic precipitation of inorganic phosphate was seen only after EDTA-Tris-sucrose exposure.
14 references, click to expand
-
Electron microscopical studies of phage multiplication. IV. The establishment of the DNA pool of vegetative phage and the maturation of phage particles.Virology. 1959 Aug;8:478-98 PMID: 14405215
-
Electron microscopy of alkaline phosphatase of Escherichia coli.Can J Microbiol. 1966 Aug;12(4):605-7 PMID: 5338879
-
A NONSPECIFIC INCREASE IN PERMEABILITY IN ESCHERICHIA COLI PRODUCED BY EDTA.Proc Natl Acad Sci U S A. 1965 Apr;53:745-50 PMID: 14324530
-
Effect of hydroxyurea on virus development. I. Electron microscopic study of the effect on the development of bacteriophage T4.J Bacteriol. 1966 Feb;91(2):823-33 PMID: 5327372
-
The cyclic phosphodiesterases (3'-nucleotidases) of the enterobacteriaceae.Biochemistry. 1968 Oct;7(10):3774-80 PMID: 4300709
-
The 5'-nucleotidase of Escherichia coli. II. Surface localization and purification of the Escherichia coli 5'-nucleotidase inhibitor.J Biol Chem. 1967 Sep 10;242(17):3905-11 PMID: 5341266
-
Release of surface enzymes in Enterobacteriaceae by osmotic shock.J Bacteriol. 1967 Dec;94(6):1934-45 PMID: 4294595
-
Location of sulfate-binding protein in Salmonella typhimurium.J Bacteriol. 1968 Oct;96(4):1049-54 PMID: 4971882
-
On the surface localization of enzymes in E. coli.Biochem Biophys Res Commun. 1964 Oct 14;17(3):215-9 PMID: 4285950
-
Effect of ethylenediaminetetraacetic acid-Tris(hydroxymethyl)aminomethane on release of the acid-soluble nucleotide pool and on breakdown of ribosomal ribonucleic acid in Escherichia coli.J Bacteriol. 1967 Apr;93(4):1360-8 PMID: 4962058
-
SITES OF ADENOSINE TRIPHOSPHATASE ACTIVITY IN BACTERIA.J Bacteriol. 1964 Oct;88:1196-8 PMID: 14219037
-
BACTERIAL PROTOPLASTS INDUCED BY PENICILLIN.Proc Natl Acad Sci U S A. 1956 Sep;42(9):574-7 PMID: 16589908
-
The 5'-nucleotidases (uridine diphosphate sugar hydrolases) of the Enterobacteriaceae.Biochemistry. 1968 Oct;7(10):3766-73 PMID: 4878706
-
The 5'-nucleotidase of Escherichia coli. I. Purification and properties.J Biol Chem. 1967 Sep 10;242(17):3896-904 PMID: 5341265
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
