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PMID: 4176224 Published · ppublish English Journal Article

Cells involved in the immune response. IV. The response of normal and immune rabbit bone marrow and lymphoid tissue lymphocytes to antigens in vitro.

The Journal of experimental medicine ·Vol. 128 ·No. 5 ·1968-11-01 ·Pages 1099-128

Singhal SK, Richter M

Abstract

Cell suspensions of immune rabbit lymph nodes and spleen were capable of undergoing blastogenesis and mitosis and of incorporating tritiated thymidine when maintained in culture with the specific antigen in vitro. They did not respond to other, non-cross-reacting antigens. The blastogenic response obtained with immune lymph node cells could be correlated with the antibody synthesizing capacity of fragment cultures prepared from the same lymph nodes. Cell suspensions of immune bone marrow responded to non-cross-reacting antigens only whereas cell suspensions of immune thymus, sacculus rotundus, and appendix did not respond when exposed to any of the antigens tested. On the other hand, neither fragments nor cell suspensions prepared from lymph nodes, spleen, and thymus of normal, unimmunized rabbits responded with antibody formation and blastogenesis when exposed to any of the antigens. However, normal bone marrow cells responded with marked blastogenesis and tritiated thymidine uptake. The specificity of this in vitro bone marrow response was demonstrated by the fact that the injection of a protein antigen in vivo resulted in the loss of reactivity by the marrow cell to that particular antigen but not to the other, non-cross-reacting antigens. Furthermore, bone marrow cells of tolerant rabbits failed to respond to the specific antigen in vitro. It was also demonstrated that normal bone marrow cells incubated with antigen are capable of forming antibody which could be detected by the fluorescent antibody technique. This response of the bone marrow cells has been localized to the lymphocyte-rich fraction of the bone marrow. It is concluded that the bone marrow lymphocyte, by virtue of its capacity to react with blastogenesis and mitosis and with antibody formation upon initial exposure to the antigen, a capacity not possessed by lymphocytes of the other lymphoid organs, has a preeminent role in the sequence of cellular events culminating in antibody formation.

MeSH Terms
Animals Antigen-Antibody Reactions Antigens Appendix/immunology Autoradiography Bone Marrow/immunology Bone Marrow Cells Cats Cattle Culture Techniques Dogs Ear, Inner/immunology Erythrocytes/immunology Fluorescent Antibody Technique Hemagglutination Tests Hemocyanins Horses Humans Immune Tolerance Immunization Lymph Nodes/immunology Lymphocytes/immunology Lymphoid Tissue/immunology Mercaptopurine/pharmacology Ovalbumin Rabbits Salmonella/immunology Serum Albumin Serum Albumin, Bovine Sheep Spleen/immunology Thymidine/metabolism Thymus Gland/immunology Tritium gamma-Globulins
Chemicals
Antigens Serum Albumin gamma-Globulins Tritium Serum Albumin, Bovine Ovalbumin Hemocyanins Mercaptopurine Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Singhal S K
Richter M
References (52)
52 references, click to expand
  1. The immunologic significance of antigen induced lymphocyte transformation in vitro.
    J Immunol. 1966 Aug;97(2):239-47 PMID: 4162289
  2. The effect of skin homograft rejection on recipient and donor mixed leukocyte cultures.
    J Exp Med. 1965 Oct 1;122(4):651-64 PMID: 5321601
  3. Specific response of human lymphocytes to pollen antigen in tissue culture.
    J Allergy. 1966 Dec;38(6):321-9 PMID: 5225108
  4. STUDIES ON ANTIBODY PRODUCTION : VI. THE COURSE, SENSITIVITY, AND HISTOLOGY OF THE SECONDARY RESPONSE IN VITRO.
    J Exp Med. 1963 Jun 1;117(6):1053-62 PMID: 19867229
  5. Number of antibody-forming cells in primary and secondary reactions after administration of antigen.
    Nature. 1967 Dec 23;216(5121):1233-5 PMID: 6076078
  6. THE DEVELOPMENT OF LARGE IMMATURE MONONUCLEAR CELLS IN MIXED LEUKOCYTE CULTURES.
    Blood. 1964 Jan;23:108-16 PMID: 14107045
  7. The X-Y-Z scheme of immunocyte maturation. II. The effect of antigen on spontaneous escape from immune paralysis.
    J Immunol. 1967 Apr;98(4):827-35 PMID: 6022895
  8. THE IN VITRO IMMUNE RESPONSE OF PERIPHERAL BLOOD LYMPHOCYTES.
    Semin Hematol. 1965 Jan;2:68-89 PMID: 14268738
  9. Cells involved in the immune response. I. The response of normal rabbit bone marrow cells to antigens in vitro.
    Int Arch Allergy Appl Immunol. 1968;33(5):493-500 PMID: 5657244
  10. Effect of x-ray dose on mortality and skin transplantability in mice receiving F1 hybrid marrow.
    Proc Soc Exp Biol Med. 1956 Oct;93(1):98-100 PMID: 13370588
  11. TISSUE CULTURE STUDIES OF THE HUMAN LYMPHOCYTE.
    Science. 1964 Dec 25;146(3652):1648-54 PMID: 14224511
  12. The X-Y-Z scheme of immunocyte maturation. 3. Early IgM memory and the nature of the memory cell.
    J Immunol. 1967 Apr;98(4):836-43 PMID: 4164560
  13. AN IN VITRO SYSTEM FOR THE STUDY OF THE MECHANISM OF ANTIGENIC STIMULATION IN THE SECONDARY RESPONSE.
    Immunology. 1964 Jan;7:40-53 PMID: 14113075
  14. The secondary antibody response in tissue culture. I. Observations on prolonged antibody synthesis by lymph node fragments in media of different composition.
    Can J Biochem. 1965 Jun;43(6):787-801 PMID: 5839217
  15. Interrelationships of myeloid and lymphoid cells: studies with chromosome-marked cells transfused into lethally irradiated mice.
    Proc R Soc Lond B Biol Sci. 1966 Jul 19;165(998):78-102 PMID: 4380152
  16. The X-Y-Z scheme of immunocyte maturation. IV. The exhaustion of memory cells.
    J Exp Med. 1968 Feb 1;127(2):307-25 PMID: 5635381
  17. Survival of lethally X-irradiated rats after treatment with isogenic marrow lymphocytes.
    Am J Physiol. 1967 Oct;213(4):923-7 PMID: 4860885
  18. Experimental treatment of total-body irradiation injury: a brief review.
    Blood. 1957 Aug;12(8):746-54 PMID: 13446002
  19. IMMUNE RESPONSE AND MITOSIS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO.
    Science. 1963 Nov 29;142(3596):1185-7 PMID: 14069243
  20. The carriage of immunological memory by small lymphocytes in the rat.
    J Exp Med. 1966 Nov 1;124(5):1017-30 PMID: 5926297
  21. Role of the thymus and other central lymphoid tissues in immunological disease.
    Annu Rev Med. 1967;18:113-38 PMID: 4164731
  22. Quantitative studies on the mixed lymphocyte interaction in rats. I. Conditions and parameters of response.
    J Exp Med. 1967 Oct 1;126(4):625-54 PMID: 6055759
  23. Current concepts of the immunological function of the thymus.
    Physiol Rev. 1967 Jul;47(3):437-520 PMID: 4864541
  24. Immunological activity of thymus and thoracic-duct lymphocytes.
    Proc Natl Acad Sci U S A. 1968 Jan;59(1):296-303 PMID: 4873344
  25. GENETIC STUDIES ON THE MIXED LEUKOCYTE REACTION.
    Science. 1964 Sep 18;145(3638):1315-6 PMID: 14173419
  26. Immunocompetence of transferred thymus-marrow cell combinations.
    J Immunol. 1966 Dec;97(6):828-32 PMID: 5333748
  27. Mechanisms of antibody production.
    Annu Rev Med. 1967;18:81-96 PMID: 5337538
  28. Autografts of bone marrow in dogs after lethal total-body radiation.
    Blood. 1960 Feb;15:255-66 PMID: 14420699
  29. The thymus and the precursors of antigen reactive cells.
    Nature. 1967 Nov 18;216(5116):659-63 PMID: 6082462
  30. Response of human blood lymphocytes to tuberculin PPD in tissue culture.
    Immunology. 1967 Apr;12(4):417-29 PMID: 6023759
  31. Immunologically stimulated human peripheral blood lymphocytes in vitro. II. Mixed lymphocyte cultures with related and unrelated donors.
    Int Arch Allergy Appl Immunol. 1966;30(2):177-89 PMID: 6005923
  32. CELLULAR TRAFFIC OF THE THYMUS: EXPERIMENTS WITH CHROMOSOME MARKERS. EVIDENCE THAT THE THYMUS PLAYS AN INSTRUCTIONAL PART.
    Nature. 1964 Feb 29;201:884-5 PMID: 14132779
  33. The fate of parental strain small lymphocytes in F1 hybrid rats.
    Ann N Y Acad Sci. 1962 Oct 24;99:432-55 PMID: 13949633
  34. The in vitro blastogenic response of lymphocytes of ragweed-sensitive individuals.
    J Allergy. 1968 Mar;41(3):140-51 PMID: 5238592
  35. The mitotic response of thymus-derived cells to antigenic stimulus.
    Transplantation. 1966 Jul;4(4):438-51 PMID: 5967867
  36. The variation in response of human peripheral lymphocytes to phytohemagglutinin in vitro.
    Int Arch Allergy Appl Immunol. 1967;32(3):288-93 PMID: 6073626
  37. LYMPHOCYTE INTERACTION: A POTENTIAL HISTOCOMPATIBILITY TEST IN VITRO.
    Science. 1964 Feb 21;143(3608):813-4 PMID: 14088078
  38. Synthesis of antibody in vitro by lymph node fragments using absorbable gelatin sponges.
    Nature. 1966 May 7;210(5036):645 PMID: 5964578
  39. Lymphocytes and transplantation immunity.
    Adv Immunol. 1967;7:189-273 PMID: 4868523
  40. Transplantation of antibody-forming cells in lethally irradiated mice.
    J Immunol. 1961 Feb;86:113-22 PMID: 13704368
  41. Cellular commitments to immune responses.
    Annu Rev Microbiol. 1967;21:181-204 PMID: 4860256
  42. The role of lymphocytes in antibody formation. I. Restoration of the haemolysin response in x-irradiated rats with lymphocytes from normal and immunologically tolerant donors.
    Proc R Soc Lond B Biol Sci. 1967 Sep 12;168(1012):229-43 PMID: 4383552
  43. ANTIGEN STIMULATED PROLIFERATION IN LYMPHOID AND MYELOID TISSUES FROM IMMUNIZED RABBITS.
    Proc Soc Exp Biol Med. 1964 Dec;117:708-11 PMID: 14244931
  44. Thymus-marrow cell combinations. Synergism in antibody production.
    Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1167-71 PMID: 5918139
  45. INHIBITION OF VIRAL REPLICATION BY ANTICELLULAR SERUM.
    Virology. 1963 Aug;20:629-35 PMID: 14059829
  46. EFFECT OF SOME ANTIGENS ON LEUCOCYTE CULTURES.
    J Pathol Bacteriol. 1965 Jan;89:63-70 PMID: 14263498
  47. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera.
    J Exp Med. 1951 Feb;93(2):107-20 PMID: 14803635
  48. Time of appearance and distribution of cells capable of secondary immune response following primary immunization.
    Immunology. 1967 Jun;12(6):675-87 PMID: 6027423
  49. In vitro lymphocyte transformation in atopic patients: induced by antigens.
    J Allergy. 1967 Feb;39(2):65-81 PMID: 4962018
  50. The immunological significance in the guinea-pig of in vitro transformation of lymph node, spleen and peripheral blood lymphocytes.
    Immunology. 1968 Feb;14(2):257-64 PMID: 5640946
  51. In vitro studies of immunological responses of lymphoid cells.
    Adv Immunol. 1967;6:253-336 PMID: 4963320
  52. The secondary antibody response in tissue culture. II. Differences in the course of antibody synthesis to diphtheria toxoid and rat serum albumin.
    Can J Biochem. 1965 Jun;43(6):803-9 PMID: 5839218
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.128.5.1099
Published
1968-11-01
Pages
1099-128
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138559
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.128.5.1099 PMC Full Text View journal details
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