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PMID: 4173472 Published · ppublish English Journal Article

Comparative studies of two types of bovine immunoglobulin G heavy chains.

The Biochemical journal ·Vol. 107 ·No. 4 ·1968-04-00 ·Pages 559-64

Milstein CP, Feinstein A

Abstract

;Fingerprints' of bovine colostrum and serum immunoglobulin G1 heavy chains were extremely similar, but different from serum immunoglobin G2 heavy chains. Serum immunoglobulin G1 and immunoglobulin G2 heavy chains were treated with cyanogen bromide. The fractions from the C-terminal end of the heavy chains were isolated and the amino acid sequence of this fraction from immunoglobulin G2 was:His-Glx-Ala-Leu-His-Asx-His-Tyr-Met-Gln-Lys-Ser-Thr-Ser-Lys-Ser-Ala-GlyThe amino acid composition of this fraction from immunoglobulin G1 was the same except for the methionine, which in immunoglobulin G1 was replaced by threonine.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Animals Cattle Colostrum/analysis Cyanides Electrophoresis Oxidation-Reduction Peptides/analysis Trypsin gamma-Globulins/analysis
Chemicals
Amino Acids Cyanides Peptides gamma-Globulins Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Milstein C P
Feinstein A
References (12)
12 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1070559
Published
1968-04-00
Pages
559-64
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1198699
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1070559 PMC Full Text View journal details
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