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PMID: 4173471 Published · ppublish English Journal Article

The effect of ribonuclease on polysomes and ribosomes of bacteria and animal cells.

The Biochemical journal ·Vol. 107 ·No. 4 ·1968-04-00 ·Pages 481-9

Fenwick ML

Abstract

The mildest treatment with ribonuclease that causes any disaggregation of the polysomes of Escherichia coli or HeLa cells simultaneously attacks the RNA of the constituent ribosomes. It is concluded that the susceptibility to ribonuclease of polysomes does not suggest that they are held together by a strand of messenger RNA. The RNA of the larger sub-unit of bacterial ribosomes has particularly sensitive regions resulting in a non-random degradation. The RNA of the smaller sub-unit of E. coli ribosomes is relatively resistant to ribonuclease attack. The same may be true of the respective sub-units of the intact HeLa-cell ribosome, but both sub-units become very sensitive to ribonuclease on dissociation from each other.

MeSH Terms
Animals Chloramphenicol Dactinomycin Escherichia coli HeLa Cells Humans Pancreas/enzymology RNA RNA, Messenger Ribonucleases/pharmacology Ribosomes/drug effects Ultracentrifugation
Chemicals
RNA, Messenger Dactinomycin RNA Chloramphenicol Ribonucleases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Fenwick M L
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40 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1070481
Published
1968-04-00
Pages
481-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1198691
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1070481 PMC Full Text View journal details
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