Studies on the effect of the carrier molecule on antihapten antibody synthesis. I. Effect of carrier on the nature of the antibody synthesized.
1. Fluorescence quenching has been calibrated, by comparison with equilibrium dialysis, for measurement of antibody-hapten association constants with guinea pig antibody to the 2,4-dinitrophenyl (DNP) determinant. The maximum quenching with all sites occupied which gave best agreement with equilibrium dialysis was found to be 100%. 2. The carrier to which the DNP-hapten is coupled influences significantly the amount, class (gamma(1) or gamma(2)), and affinity of the antibody of DNP specificity made by individual guinea pigs. 3. The affinities of gamma(1)- and gamma(2)-antibodies from individual guinea pigs are generally very similar. 4. Precipitin curves with gamma(1)-antibodies show more marked inhibition of precipitation in antigen excess than do precipitin curves with the less charged gamma(2)-antibodies, indicating the importance of nonspecific forces in the precipitation reactions.
27 references, click to expand
-
EXCITATION ENERGY TRANSFER AND THE QUANTITATIVE STUDY OF THE ANTIBODY HAPTEN REACTION.Proc Natl Acad Sci U S A. 1960 Nov;46(11):1470-82 PMID: 16590773
-
A study of the distribution of 2,4-dinitrobenzene sensitizers between isolated lymph node cells and extracellular medium in relation to induction of contact skin sensitivity.J Exp Med. 1959 Aug 1;110(2):187-206 PMID: 13673134
-
STUDIES ON THE PROPERTIES OF FRAGMENTS OF GUINEA PIG GAMMA-1 AND GAMMA-2 ANTIBODIES OBTAINED BY PAPAIN DIGESTION AND MILD REDUCTION.J Immunol. 1964 Dec;93:1008-14 PMID: 14241758
-
VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE.Biochemistry. 1964 Jul;3:996-1008 PMID: 14214095
-
THE IMMUNOGENICITY OF HAPTEN-POLYLYSINE CONJUGATES. A COMPARISON OF D AND L POLYMERS.J Immunol. 1965 Feb;94:289-94 PMID: 14265900
-
Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies.J Exp Med. 1963 Jun 1;117:937-49 PMID: 13967257
-
Zone electrophoresis.Methods Biochem Anal. 1954;1:141-70 PMID: 13193528
-
Studies on the dimensions of the rabbit antibenzylpenicilloyl antibody-combing sites.J Exp Med. 1963 Jan 1;117:161-83 PMID: 13930142
-
QUANTITATIVE STUDIES ON THE PRECIPITATION OF ANTI-2,4DINITROPHENYL ANTIBODY IN ANTIGEN EXCESS.J Immunol. 1964 May;92:702-8 PMID: 14170411
-
Studies on the effect of the carrier molecule on antihapten antibody synthesis. II. Carrier specificity of anti-2,4-dinitrophenyl-poly-l-lysine antibodies.J Exp Med. 1966 Apr 1;123(4):689-705 PMID: 4160399
-
ANTIGENICITY OF HEPTEN CONJUGATES OF POLY-D-LYSINE AND OF POLY-L-LYSINE IN STRAIN 2 GUINEA PIGS.Nature. 1964 Jun 6;202:1008-9 PMID: 14197301
-
Immunological specificity of delayed and immediate hypersensitivity reactions.J Exp Med. 1962 May 1;115:1023-36 PMID: 13867017
-
Studies of hypersensitivity to low molecular weight substances. III. The 2,4-dinitrophenyl group as a determinant in the preciptin reaction.J Immunol. 1954 Nov;73(5):296-308 PMID: 13212051
-
Studies on hypersensitivity. IV. The relationship between contact and delayed sensitivity: a study of the specificity of cellular immune reactions.J Exp Med. 1961 Mar 1;113:571-85 PMID: 13704282
-
The preparation and some properties of purified antibody specific for the 2,4-dinitrophenyl group.J Exp Med. 1960 Dec 1;112:1195-210 PMID: 13698150
-
The hydrolysis of rabbit y-globulin and antibodies with crystalline papain.Biochem J. 1959 Sep;73:119-26 PMID: 14434282
-
HEXOSE CONTENT OF GUINEA PIG GAMMA-1 AND GAMMA-2 IMMUNOGLOBULINS.Proc Soc Exp Biol Med. 1965 Feb;118:336-42 PMID: 14268615
-
ANTIGENIC RELATIONSHIP BETWEEN TWO TYPES OF 7 S GUINEA PIG GAMMA-GLOBULINS.J Immunol. 1963 Nov;91:670-6 PMID: 14089773
-
Heterogeneity of antibody sites in their relative combining affinities for structurally related haptens.J Immunol. 1958 Aug;81(2):126-35 PMID: 13563890
-
Heterogeneity and average combining constants of antibodies from individual rabbits.J Immunol. 1958 Jun;80(6):417-28 PMID: 13549727
-
STUDIES ON THE IMMUNOLOGICAL MECHANISMS OF PENICILLIN ALLERGY. II. ANTIGENIC SPECIFICITIES OF ALLERGIC WHEAL-AND-FLARE SKIN RESPONSES IN PATIENTS WITH HISTORIES OF PENICILLIN ALLERGY.Immunology. 1964 Sep;7:542-56 PMID: 14210764
-
DETERMINATION OF ANTIBODY AFFINITY FOR HAPTENS AND ANTIGENS BY MEANS OF FLUORESCENCE QUENCHING.Methods Med Res. 1964;10:115-21 PMID: 14284903
-
Studies on artifical antigens. I. Antigenicity of DNP-polylysine and DNP copolymer of lysine and glutamic acid in guinea pigs.J Exp Med. 1963 Jan 1;117:55-69 PMID: 14030666
-
EQUILIBRIUM DIALYSIS FOR MEASUREMENT OF ANTIBODY-HAPTEN AFFINITIES.Methods Med Res. 1964;10:106-14 PMID: 14284902
-
STUDIES ON ARTIFICIAL ANTIGENS. III. THE GENETIC CONTROL OF THE IMMUNE RESPONSE TO HAPTEN-POLY-L-LYSINE CONJUGATES IN GUINEA PIGS.J Exp Med. 1963 Dec 1;118:953-7 PMID: 14112274
-
STUDIES ON DELAYED HYPERSENSITIVITY. I. INFERENCES ON THE COMPARATIVE BINDING AFFINITIES OF ANTIBODIES MEDIATING DELAYED AND IMMEDIATE HYPERSENSITIVITY REACTIONS IN THE GUINEA PIG.J Exp Med. 1965 Jun 1;121:873-88 PMID: 14319404
-
SPECIFICITY OF GUINEA-PIG ANTIBODIES AND DELAYED HYPERSENSITIVITY.Nature. 1965 Jan 16;205:299-300 PMID: 14270726
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
