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PMID: 4160234 Published · ppublish English Journal Article

Nonabsorbable rabbit anti-Salmonella typhimurium antibody as detected by the complement-mediated bactericidal reaction.

Journal of bacteriology ·Vol. 91 ·No. 4 ·1966-04-00 ·Pages 1548-55

Herzberg M, Kenny KV, Robbins JB

Abstract

Herzberg, Mendel (University of Florida, Gainesville), Kathryn V. Kenny, and John B. Robbins. Nonabsorbable rabbit anti-Salmonella typhimurium antibody as detected by the complement-mediated bactericidal reaction. J. Bacteriol. 91:1548-1555. 1966.-A portion of antibody active in the complement-mediated bactericidal reaction against Salmonella typhimurium from hyperimmune rabbit serum has been shown to be nonabsorbable by repeated serial absorptions with whole heat-killed or living bacteria. The first two absorptions remove 90 to 95% of the activity, but 1 to 5% cannot be removed by subsequent absorptions. The nonabsorbable antibody appears to be a macroglobulin by density-gradient centrifugation and by comparison of activity and absorbability of purified gamma-M and gamma-G immune globulins. Alternative hypotheses involving low avidity antibody or antibody to minor cell antigenic components are offered in explanation of the phenomenon.

MeSH Terms
Absorption Animals Antibodies Blood Bactericidal Activity Complement System Proteins Hot Temperature Immune Sera Immunization In Vitro Techniques Macroglobulins Rabbits Salmonella typhimurium Ultracentrifugation gamma-Globulins
Chemicals
Antibodies Immune Sera Macroglobulins gamma-Globulins Complement System Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Herzberg M
Kenny K V
Robbins J B
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21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.91.4.1548-1555.1966
Published
1966-04-00
Pages
1548-55
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC316076
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.91.4.1548-1555.1966 PMC Full Text View journal details
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