Marking single neurons by staining with intracellular recording microelectrodes.

Science (New York, N.Y.) ·Vol. 151 ·No. 3717 ·1966-03-25 ·Pages 1538-9

Thomas RC, Wilson VJ

Abstract

Glass microelectrodes filled with a saturated solution of methyl blue or fast green in 1.0M potassium acetate can be used to mark penetrated ne. urons from which intracellular recordings have been made. Many cells can be marked in one experiment and can easily be located in subsequent histologic section.

MeSH Terms

Animals Electrophysiology Histological Techniques In Vitro Techniques Methylene Blue Motor Neurons/physiology Staining and Labeling

Chemicals

Methylene Blue

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Thomas R C

Wilson V J

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.151.3717.1538

Published

1966-03-25

Pages

1538-9

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.151.3717.1538 View journal details

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