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PMID: 415307 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Combined immunofluorescence and high-voltage electron microscopy of cultured mammalian cells, using an antibody that binds to glutaraldehyde-treated tubulin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 1 ·1978-01-00 ·Pages 334-8

Eckert BS, Snyder JA

Abstract

An antibody against tubulin that binds specifically to microtubules in glutaraldehyde-fixed cells has been prepared. Sodium dodecyl sulfate-denatured tubulin treated with glutaraldehyde was used to immunize rabbits. In glutaraldehyde-fixed cells the fluorescent image of this antibody reveals a fine lattice of microtubules around the nucleus of PtK1 (Potorous tridactylis) cells and many uniformly fluorescent microtubules in the peripheral cytoplasm. In cells fixed with formaldehyde the microtubules appear to have a similar distribution, but the fluorescent image is much less uniform. Combined high-voltage electron microscopy and immunofluorescent studies reveal that microtubules are found in the cytoplasm in the same region as the fluorescent antibody stain.

MeSH Terms
Antibody Specificity Cell Nucleus/ultrastructure Cells, Cultured Cold Temperature Cytoplasm/ultrastructure Demecolcine/pharmacology Fluorescent Antibody Technique Glutaral/pharmacology Glycoproteins/immunology Microtubules/drug effects,ultrastructure Tubulin/immunology
Chemicals
Glycoproteins Tubulin Glutaral Demecolcine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Eckert B S
Snyder J A
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.1.334
Published
1978-01-00
Pages
334-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411242
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.1.334 PMC Full Text View journal details
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