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PMID: 41517 Published · ppublish English Journal Article

Intracellular localization of long-chain acyl-coenzyme A hydrolase and acyl-L-carnitine hydrolase in brown adipose tissue from guinea pigs.

The Biochemical journal ·Vol. 182 ·No. 2 ·1979-08-15 ·Pages 347-51

Berge RK, Slinde E, Farstad M

Abstract

The activities of long-chain acyl-CoA hydrolase (palmitoyl-CoA hydrolase, EC 3.1.2.2) and long-chain acyl-L-carnitine hydrolase, EC 3.1.1.28) in brown adipose tissue from cold-exposed and control guinea pigs were studied. Mitochondria from cold-exposed animals hydrolysed 21 nmol of palmitoyl-CoA/min per mg of protein and 1.3 nmol of palmitoyl-L-carnitine/min per mg of protein, and the specific activities were respectively 2 and 5 times as high in cold-exposed as in control animals. The subcellular-localization studies showed that both the long-chain acyl-CoA hydrolase and long-chain acyl-L-carnitine hydrolase were localized in the mitochondria. A location also in the soluble fraction cannot be excluded. The long-chain acyl-CoA hydrolase activity was doubled when the mitochondria were disrupted; this indicates that the enzyme is localized in the matrix compartment.

MeSH Terms
Adipose Tissue, Brown/enzymology Animals Carboxylic Ester Hydrolases/metabolism Cold Temperature Guinea Pigs Mitochondria/enzymology Palmitoyl-CoA Hydrolase/metabolism Palmitoylcarnitine Subcellular Fractions/enzymology Thiolester Hydrolases/metabolism
Chemicals
Palmitoylcarnitine Carboxylic Ester Hydrolases Thiolester Hydrolases Palmitoyl-CoA Hydrolase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Berge R K
Slinde E
Farstad M
References (29)
29 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1820347
Published
1979-08-15
Pages
347-51
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1161313
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1820347 PMC Full Text View journal details
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