The colony forming efficiency of single cells and cell aggregates from a spontaneous mouse mammary tumour using the lung colony assay.

British journal of cancer ·Vol. 30 ·No. 4 ·1974-10-00 ·Pages 332-6

Thompson SC

Abstract

Single cell suspensions of spontaneous C3H mammary tumours did not produce colonies in the lungs of syngeneic mice after intravenous injection whereas cell aggregates obtained from similar tumours did. The number of colonies formed after the injection of aggregates was related to aggregate size and was proportional to the number injected.Single cells grew in an intramuscular site and in vitro and it was also found that if spontaneous tumours were passaged in vivo a few times single cells would grow in the lung.

MeSH Terms

Animals Cell Division Cell Separation Cells, Cultured Clone Cells Female Filtration Injections, Intramuscular Injections, Intravenous Lung Neoplasms/pathology Male Mammary Neoplasms, Experimental/pathology Methods Mice Mice, Inbred C3H Neoplasm Metastasis/etiology Neoplasm Transplantation Staining and Labeling

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Thompson S C

References (6)

6 references, click to expand

A lung-colony assay to determine the radiosensitivity of cells of a solid tumour.
Int J Radiat Biol Relat Stud Phys Chem Med. 1969 Jul;15(5):435-44 PMID: 5306346
The fate of circulating methylcholanthrene tumor cells in mice with tumor-specific immunity.
Cancer. 1971 Sep;28(3):641-6 PMID: 5096927
Influence of preirradiation of lung on development of artificial pulmonary metastases of fibrosarcoma in mice.
Cancer Res. 1973 Aug;33(8):1931-6 PMID: 4720801
Lung colony assays of murine mammary tumor cells irradiated in vivo and in vitro.
Radiology. 1973 Dec;109(3):703-6 PMID: 4772186
The effect of lung irradiation on the incidence of pulmonary metastases in mice.
Br J Radiol. 1973 Aug;46(548):613-8 PMID: 4783058
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Article Info

Journal

British journal of cancer

Abbr.

Br J Cancer

ISSN

0007-0920

DOI

10.1038/bjc.1974.201

Published

1974-10-00

Pages

332-6

Language

English

Region

England

NLM ID

0370635

PMCID

PMC2009284

Subset

External Links

PubMed source DOI: 10.1038/bjc.1974.201 PMC Full Text View journal details

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The colony forming efficiency of single cells and cell aggregates from a spontaneous mouse mammary tumour using the lung colony assay.

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