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PMID: 4140514 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Studies on the reverse transcriptase of RNA tumor viruses. Structural relatedness of two subunits of avian RNA tumor viruses.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 12 ·1974-12-00 ·Pages 4991-4

Gibson W, Verma IM

Abstract

The structural relationship of the small (alpha) and large (beta) subunits of reverse transcriptase isolated from two avian RNA tumor viruses has been examined by tryptic peptide analysis. Comparison of the tryptic hydrolysates of the isolated subunits by two-dimensional separation on thin-layer cellulose plates indicates that (i) the alpha subunit of reverse transcriptase of avian myeloblastosis virus is structurally related to the beta subunit; (ii) the alpha and beta subunits of the enzyme of Rous sarcoma virus also appear to be related; and (iii) there appears to be an extensive amino-acid sequence homology between reverse transcriptases of avian myeloblastosis virus and Rous sarcoma virus. Evidence is also presented that both alpha and beta subunits can be identified in purified avian myeloblastosis virions.

MeSH Terms
Amino Acid Sequence Autoradiography Avian Leukosis Virus/enzymology Avian Myeloblastosis Virus/enzymology Avian Sarcoma Viruses/enzymology Electrophoresis, Polyacrylamide Gel Hydrolysis Iodine Radioisotopes Peptide Fragments/analysis Protein Conformation RNA-Directed DNA Polymerase/analysis Trypsin
Chemicals
Iodine Radioisotopes Peptide Fragments RNA-Directed DNA Polymerase Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gibson W
Verma I M
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.12.4991
Published
1974-12-00
Pages
4991-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC434025
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.12.4991 PMC Full Text View journal details
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