Enzymatic synthesis of solid phase-bound DNA sequences corresponding to specific mammalian genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 10 ·1974-10-00 ·Pages 3892-5

Venetianer P, Leder P

Abstract

With oligo(dT)-cellulose as primer, RNA-dependent DNA polymerase catalyzes the synthesis of cellulose-bound DNA that is complementary to mouse globin mRNA. The resulting cellulose-bound or solid phase complementary DNA hybridizes specifically with globin mRNA and permits the recovery of intact globin mRNA. This simple technique for the synthesis of solid phase-bound complementary DNA provides an additional and convenient method for the purification of specific genetic sequences.

MeSH Terms

Animals Avian Myeloblastosis Virus/enzymology Base Sequence Cellulose Centrifugation, Density Gradient DNA/analysis,biosynthesis Genes Globins Immunoglobulins In Vitro Techniques Iodine Radioisotopes Mice Nucleic Acid Hybridization Oligonucleotides RNA, Messenger/analysis RNA-Directed DNA Polymerase/metabolism Templates, Genetic Thymine Nucleotides

Chemicals

Immunoglobulins Iodine Radioisotopes Oligonucleotides RNA, Messenger Thymine Nucleotides Globins Cellulose DNA RNA-Directed DNA Polymerase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Venetianer P

Leder P

References (11)

11 references, click to expand

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In vitro synthesis of DNA complementary to mRNA derived from a light chain-producing myeloma tumour.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.10.3892

Published

1974-10-00

Pages

3892-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC434291

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.10.3892 PMC Full Text View journal details

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Enzymatic synthesis of solid phase-bound DNA sequences corresponding to specific mammalian genes.

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