Measurement of intracellular iodophilic polysaccharide in two cariogenic strains of Streptococcus mutans by cytochemical and chemical methods.

Infection and immunity ·Vol. 10 ·No. 3 ·1974-09-00 ·Pages 597-604

DiPersio JR, Mattingly SJ, Higgins ML, Shockman GD

Abstract

Quantitative cytological and chemical methods have been developed to study the intracellular iodophilic polysaccharide (IPS) content of two strains of Streptococcus mutans. The cytological method uses a periodic acid-chlorite treatment of thin sections to increase the affinity of IPS for uranyl and lead salts. This results in the IPS appearing as individual electron-dense granules which can be counted for quantitative studies. As a basis for these quantitative studies, IPS was measured chemically by dissolving whole cells with hot KOH and quantitating spectrophotometrically the amount of iodine-polysaccharide complex formed.

MeSH Terms

Bacteriological Techniques Culture Media Densitometry Glycogen/isolation & purification Iodine Microscopy, Electron Periodic Acid Polysaccharides, Bacterial/analysis Potassium Iodide Staining and Labeling Streptococcus/analysis,ultrastructure

Chemicals

Culture Media Polysaccharides, Bacterial Periodic Acid Potassium Iodide Glycogen Iodine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

DiPersio J R

Mattingly S J

Higgins M L

Shockman G D

References (14)

14 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.10.3.597-604.1974

Published

1974-09-00

Pages

597-604

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC422994

Subset

External Links

PubMed source DOI: 10.1128/iai.10.3.597-604.1974 PMC Full Text View journal details

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Measurement of intracellular iodophilic polysaccharide in two cariogenic strains of Streptococcus mutans by cytochemical and chemical methods.

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