Cell-cycle analysis in 20 minutes.

Science (New York, N.Y.) ·Vol. 184 ·No. 4143 ·1974-06-21 ·Pages 1297-8

Crissman HA, Tobey RA

Abstract

Mithramycin added to mammalian cells fixed in aqueous ethanol is bound to DNA and fluoresces in direct proportion to the cellular DNA content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides a rapid method for cell-cycle analysis and for the first time permits continuous monitoring of cell-cycle kinetics during ongoing experiments.

MeSH Terms

Animals Cell Division Cell Line Cells, Cultured/analysis Cricetinae Cytological Techniques DNA/analysis DNA Replication Female Fluorescence Fluorometry HeLa Cells Humans Kinetics Lung/embryology Methods Mitosis Ovary Plicamycin Spectrometry, Fluorescence Staining and Labeling

Chemicals

DNA Plicamycin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Crissman H A

Tobey R A

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.184.4143.1297

Published

1974-06-21

Pages

1297-8

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.184.4143.1297 View journal details

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