A simple method for detecting mycoplasma infection of cell cultures.

Journal of clinical pathology ·Vol. 27 ·No. 1 ·1974-01-00 ·Pages 70-3

Harnett GB, Phillips PA, Mackay-Scollay EM

Abstract

This paper describes the overnight Giemsa staining method for detecting mycoplasma infection of cell cultures. This simple method requires no special equipment and has practical application in most cell culture laboratories. This technique has been used conveniently to monitor stock cell cultures for mycoplasma infection over a 12-month period avoiding any contamination.

MeSH Terms

Amnion Buffers Cell Line Cells, Cultured Culture Media Fluoresceins Humans Methods Microscopy Mycoplasma/growth & development,isolation & purification Phenothiazines Staining and Labeling

Chemicals

Buffers Culture Media Fluoresceins Phenothiazines

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Harnett G B

Phillips P A

Mackay-Scollay E M

References (18)

18 references, click to expand

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Article Info

Journal

Journal of clinical pathology

Abbr.

J Clin Pathol

ISSN

0021-9746

DOI

10.1136/jcp.27.1.70

Published

1974-01-00

Pages

70-3

Language

English

Region

England

NLM ID

0376601

PMCID

PMC477990

Subset

External Links

PubMed source DOI: 10.1136/jcp.27.1.70 PMC Full Text View journal details

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