Human DNA polymerase 3 (R-DNA polymerase): distinction from DNA polymerase I and reverse transcriptase.

Science (New York, N.Y.) ·Vol. 183 ·No. 4127 ·1974-03-01 ·Pages 867-9

Lewis BJ, Abrell JW, Smith RG, Gallo RC

Abstract

DNA polymerase III is an enzyme activity in eukaryotic cells which under certain conditions shows strong preference for polyadenylic acid as template when primed by oligodeoxythymidylate. Its first complete separation from other DNA polymerases in human lymphoblasts is reported. This enzyme is biochemically and immunologically distinct from DNA polymerase I and from viral reverse transcriptase from a primtate type C virus.

MeSH Terms

Adenine Nucleotides Animals Chromatography, DEAE-Cellulose Cytosine Nucleotides DNA DNA Nucleotidyltransferases/analysis,classification,isolation & purification,metabolism Epitopes Guanine Nucleotides Humans Immunoassay Lymphocytes/enzymology Polynucleotides RNA, Viral RNA-Directed DNA Polymerase/metabolism Rats/immunology Retroviridae/enzymology Templates, Genetic Thymine Nucleotides/metabolism Transcription, Genetic Tritium

Chemicals

Adenine Nucleotides Cytosine Nucleotides Epitopes Guanine Nucleotides Polynucleotides RNA, Viral Thymine Nucleotides Tritium DNA DNA Nucleotidyltransferases RNA-Directed DNA Polymerase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Lewis B J

Abrell J W

Smith R G

Gallo R C

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.183.4127.867

Published

1974-03-01

Pages

867-9

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.183.4127.867 View journal details

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Human DNA polymerase 3 (R-DNA polymerase): distinction from DNA polymerase I and reverse transcriptase.

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