Abstract
Virus-like particles were purified from a single nonaflatoxin-producing isolate of Aspergillus flavus. The virus-like particles were spherical, measuring 27 to 30 nm in diameter, were electrophoretically homogeneous, and sedimented at approximately 49S. The particles had a buoyant density of 1.28 g/cm(3) in CsCl and contained no detectable nucleic acid.
MeSH Terms
Aflatoxins/biosynthesis
Aspergillus flavus/metabolism
Cell Fractionation
Centrifugation, Density Gradient
Chromatography, Thin Layer
Electrophoresis, Polyacrylamide Gel
Immunodiffusion
Inclusion Bodies, Viral
Microscopy, Electron
Nucleoproteins/analysis
Phosphotungstic Acid
Spectrophotometry, Ultraviolet
Staining and Labeling
Viral Proteins/analysis
Chemicals
Aflatoxins
Nucleoproteins
Viral Proteins
Phosphotungstic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wood H A
Bozarth R F
Adler J
Mackenzie D W
References (12)
12 references, click to expand
-
The Penicillium stoloniferum virus complex: two similar double-stranded RNA virus-like particles in a single cell.
Virology. 1971 Aug;45(2):516-23
PMID: 4106353
-
Viruslike particles associated with an isolate of penicillium brevi-compactum.
Virology. 1971 Jun;44(3):592-8
PMID: 4109520
-
Bacteriophages of Clostridium botulinum.
Can J Microbiol. 1972 Jan;18(1):67-76
PMID: 4110830
-
Properties of viruslike particles of Penicillium chrysogenum: one double-stranded RNA molecule per particle.
Virology. 1972 Mar;47(3):604-9
PMID: 4622292
-
Studies on the virulence of bacteriophage-infected strains of Corynebacterium diphtheriae.
J Bacteriol. 1951 Jun;61(6):675-88
PMID: 14850426
-
Buoyant density changes of cowpea mosaic virus components at different pH values.
Virology. 1971 Feb;43(2):511-3
PMID: 5543838
-
Photometric scanning of centrifuged density gradient columns.
Anal Biochem. 1963 Apr;5:271-83
PMID: 14014902
-
Purification and properties of the replicative intermediate of the RNA bacteriophage R17.
Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504-11
PMID: 5227669
-
Diversity of RNA components in green plant tissues.
Nature. 1967 Jul 22;215(5099):363-7
PMID: 6058288
-
Virus degradation and nucleic acid release in single-phase phenol systems.
Arch Biochem Biophys. 1968 Mar 20;124(1):401-12
PMID: 5661612
-
Wound tumor virus: purification and fractionation of the double-stranded ribonucleic acid.
Virology. 1970 Feb;40(2):329-34
PMID: 4314869
-
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75
PMID: 14907713