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PMID: 412789 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cellular immunity to bacteria: impairment of in vitro lymphocyte responses to Pseudomonas aeruginosa in cystic fibrosis patients.

Infection and immunity ·Vol. 18 ·No. 3 ·1977-12-00 ·Pages 735-40

Sorensen RU, Stern RC, Polmar SH

Abstract

Lymphocyte responses to the mitogens phytohemagglutinin and concanavalin A and to Streptococcus pyogenes, Staphylococcus aureus, Haemophilus influenzae, and Pseudomonas aeruginosa were evaluated in patients with cystic fibrosis and in normal individuals. Lymphocyte proliferation in vitro was stimulated by gentamicin-killed whole bacteria, and the proliferative response was measured by [3H]thymidine incorporation. The in vitro lymphocyte responses to antibiotic-killed bacterial reached maximum thymidine incorporation after 5 days in culture and followed a unimodal dose-response curve for each of the bacteria studied. A significant specific incapacity to respond to P. aeruginosa was detected in cystic fibrosis patients with advanced clinical disease.

MeSH Terms
Antibodies, Bacterial Antigens, Bacterial Concanavalin A Cystic Fibrosis/immunology Haemophilus influenzae/immunology Humans Lectins Lymphocyte Activation Pseudomonas aeruginosa/immunology Staphylococcus aureus/immunology Streptococcus pyogenes/immunology
Chemicals
Antibodies, Bacterial Antigens, Bacterial Lectins Concanavalin A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sorensen R U
Stern R C
Polmar S H
References (20)
20 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.18.3.735-740.1977
Published
1977-12-00
Pages
735-40
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC421296
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.18.3.735-740.1977 PMC Full Text View journal details
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