Selective enrichment for genetic markers in DNA released by competent cultures of Bacillus subtilis.

Molecular & general genetics : MGG ·Vol. 155 ·No. 2 ·1977-10-20 ·Pages 179-83

Crabb WD, Streips UN, Doyle RJ

Abstract

Deoxyribonucleic acid is released into the growth medium by Bacillus subtilis at the time of competence. This DNA is enriched for the genetic markers which have previously been demonstrated to be elevated in membrane-DNA preparations and more recently in cell wall-DNA complexes. Furthermore, the purA16/leu-8 relative marker enrichment varies with time, reaching its highest point at the time of maximal competence. Enrichment remains elevated for at least 60 min further in the competence regimen. Thr results suggest that certain genetic markers of the B. subtilis chromosome are preferentially more available to the external medium as the development of competence proceeds.

MeSH Terms

Bacillus subtilis/metabolism Cell Wall/metabolism Chromosomes, Bacterial DNA Replication DNA, Bacterial/metabolism Genes Transformation, Bacterial

Chemicals

DNA, Bacterial

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Crabb W D

Streips U N

Doyle R J

References (13)

13 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00393157

Published

1977-10-20

Pages

179-83

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00393157

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Selective enrichment for genetic markers in DNA released by competent cultures of Bacillus subtilis.

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