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PMID: 4117961 Published · ppublish English Journal Article

Sendai virus-induced transcriptase from infected cells: polypeptides in the transcriptive complex.

Journal of virology ·Vol. 10 ·No. 5 ·1972-11-00 ·Pages 1037-43

Stone HO, Kingsbury DW, Darlington RW

Abstract

A Sendai virus-induced transcriptase-template complex was isolated from the cytoplasm of infected cells by combined sedimentation and isopycnic centrifugation. This transcriptive complex banded at 1.27 g/cm(3) in D(2)O-sucrose gradients. It contained two polypeptides, the viral nucleocapsid structure unit (molecular weight, 60,000) and the largest virion polypeptide (molecular weight, 75,000). The buoyant density, chemical composition, and electron microscopic appearance of the transcriptive complex indicate a structure like that of viral nucleocapsids.

MeSH Terms
Animals Carbon Isotopes Cell-Free System Cells, Cultured Centrifugation, Density Gradient Chick Embryo Cytoplasm/enzymology DNA-Directed RNA Polymerases/isolation & purification,metabolism Deuterium Electrophoresis, Polyacrylamide Gel Genetics, Microbial Lung Microscopy, Electron Molecular Weight Nucleoproteins/analysis Oxides Parainfluenza Virus 1, Human/analysis Peptides/analysis Staining and Labeling Sucrose Templates, Genetic Transcription, Genetic Tritium Viral Proteins/analysis
Chemicals
Carbon Isotopes Nucleoproteins Oxides Peptides Viral Proteins Tritium Sucrose Deuterium DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stone H O
Kingsbury D W
Darlington R W
References (18)
18 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.10.5.1037-1043.1972
Published
1972-11-00
Pages
1037-43
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356575
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.10.5.1037-1043.1972 PMC Full Text View journal details
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