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PMID: 4117771 Published · ppublish English Journal Article

Stimulated normal human lymphocytes contain a ribonuclease-sensitive DNA polymerase distinct from viral RNA-directed DNA polymerase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 11 ·1972-11-00 ·Pages 3228-32

Bobrow SN, Smith RG, Reitz MS, Gallo RC

Abstract

Ribonuclease-sensitive DNA synthesis is demonstrated in a cytoplasmic particulate fraction of normal human blood lymphocytes stimulated with phytohemagglutinin, but not in unstimulated lymphocytes. DNA polymerase purified from this fraction does not transcribe the heteropolymeric regions of 70S RNA from RNA tumor viruses, thus distinguishing this enzyme from the RNA-directed DNA polymerase (reverse transcriptase) found in oncogenic RNA viruses and human leukemic cells.

MeSH Terms
Centrifugation, Density Gradient Cytoplasm/enzymology DNA/analysis,biosynthesis DNA Nucleotidyltransferases/isolation & purification,metabolism Dactinomycin/pharmacology Humans Lectins Lymphocyte Activation Lymphocytes/drug effects,enzymology Nucleic Acid Hybridization Nucleotides/metabolism RNA, Viral/metabolism RNA-Directed DNA Polymerase Ribonucleases/metabolism Rifampin/pharmacology Templates, Genetic
Chemicals
Lectins Nucleotides RNA, Viral Dactinomycin DNA DNA Nucleotidyltransferases RNA-Directed DNA Polymerase Ribonucleases Rifampin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bobrow S N
Smith R G
Reitz M S
Gallo R C
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.11.3228
Published
1972-11-00
Pages
3228-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389742
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.11.3228 PMC Full Text View journal details
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