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PMID: 4117346 Published · ppublish English Journal Article

Major group-specific protein of rat type C viruses.

Journal of virology ·Vol. 10 ·No. 4 ·1972-10-00 ·Pages 746-50

Oroszlan S, Bova D, Huebner RJ, Gilden RV

Abstract

The major internal protein of a rat type C virus pseudotype of murine sarcoma virus, MSV(RaLV), was purified by isoelectric focusing (pI = 8.6) and used to prepare antibody in guinea pigs. The protein was identified by its reaction with antisera reactive with the mammalian type C virus group-specific (gs) antigenic determinant, gs-3. The guinea pig antisera mainly contained species-specific (gs-1) antibody for reactions in gel diffusion with other type C viruses were limited to those of rat origin, whereas in complement fixation tests heterologous reactions could be eliminated by use of appropriate antiserum concentrations without affecting homologous reactions. Guinea pig antisera against mouse, hamster, or cat gs-1 determinants did not react with MSV(RaLV) purified gs protein or with any of several other rat type C viruses.

MeSH Terms
Animals Antigens, Viral/isolation & purification Cell Line Centrifugation, Density Gradient Complement Fixation Tests Electrophoresis, Polyacrylamide Gel Epitopes Female Gammaretrovirus/immunology Guinea Pigs/immunology Helper Viruses/immunology Immune Sera Immunodiffusion Isoelectric Focusing Leucine Leukemia Virus, Feline/immunology Leukemia Virus, Murine/immunology Neoplasms, Experimental Rats/immunology Retroviridae/immunology,isolation & purification Surface-Active Agents Tritium Viral Proteins
Chemicals
Antigens, Viral Epitopes Immune Sera Surface-Active Agents Viral Proteins Tritium Leucine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Oroszlan S
Bova D
Huebner R J
Gilden R V
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19 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.10.4.746-750.1972
Published
1972-10-00
Pages
746-50
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356529
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.10.4.746-750.1972 PMC Full Text View journal details
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