Distribution of saccharide residues on membrane fragments from a myeloma-cell homogenate: its implications for membrane biogenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 10 ·1972-10-00 ·Pages 2945-9

Hirano H, Parkhouse B, Nicolson GL, Lennox ES, Singer SJ

Abstract

Ferritin conjugates of two plant agglutinins, concanavalin A and ricin, have been used as specific electron microscopic stains for covalently-bound saccharide residues on membrane fragments from a myeloma-cell homogenate. The results indicate that different saccharide residues are uniformly localized to a single surface of each membrane fragment. In particular, the ferritin-concanavalin A conjugate binds exclusively to the cisternal side of membrane fragments of the rough endoplasmic reticulum. If it is postulated that the biogenesis of eukaryotic plasma membranes involves an assembly-line process from precursor intracellular membranes, these observed asymmetric distributions of saccharides on cell membranes can be explained.

MeSH Terms

Animals Cell Line Cell Membrane/metabolism Concanavalin A Ferritins Mice Mice, Inbred BALB C Microscopy, Electron Multiple Myeloma/pathology Myeloma Proteins/metabolism Oligosaccharides/metabolism Plasmacytoma/metabolism Protein Binding Ricin Staining and Labeling

Chemicals

Myeloma Proteins Oligosaccharides Concanavalin A Ferritins Ricin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Hirano H

Parkhouse B

Nicolson G L

Lennox E S

Singer S J

References (24)

24 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.10.2945

Published

1972-10-00

Pages

2945-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389680

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.10.2945 PMC Full Text View journal details

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Distribution of saccharide residues on membrane fragments from a myeloma-cell homogenate: its implications for membrane biogenesis.

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