Properties of maedi nucleic acid and the presence of ribonucleic acid- and deoxyribonucleic acid-dependent deoxyribonucleic acid polymerase in the virions.

Journal of virology ·Vol. 10 ·No. 2 ·1972-08-00 ·Pages 228-33

Lin FH, Thormar H

Abstract

Maedi virus contains a ribonucleic acid (RNA) which can be resolved into three major components, namely, 62S, 33S, and 13S, by sucrose gradient centrifugation. The presence of RNA- and deoxyribonucleic acid (DNA)-dependent DNA polymerase in virions of maedi virus was demonstrated. The enzyme product could be converted into acid-soluble form by pancreatic deoxyribonuclease, but was resistant to digestion by pancreatic ribonuclease and to hydrolysis by NaOH.

MeSH Terms

Animals Centrifugation, Density Gradient Choroid Plexus Culture Techniques DNA, Viral/biosynthesis Hydrolysis Pulmonary Adenomatosis, Ovine/microbiology RNA, Viral/analysis,isolation & purification RNA-Directed DNA Polymerase/analysis,metabolism Ribonucleases Sheep Sodium Hydroxide Thymidine/metabolism Thymine Nucleotides/metabolism Tritium Uridine/metabolism Viruses, Unclassified/analysis,enzymology,growth & development,metabolism

Chemicals

DNA, Viral RNA, Viral Thymine Nucleotides Tritium Sodium Hydroxide RNA-Directed DNA Polymerase Ribonucleases Thymidine Uridine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lin F H

Thormar H

References (16)

16 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.10.2.228-233.1972

Published

1972-08-00

Pages

228-33

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356454

Subset

External Links

PubMed source DOI: 10.1128/JVI.10.2.228-233.1972 PMC Full Text View journal details

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Properties of maedi nucleic acid and the presence of ribonucleic acid- and deoxyribonucleic acid-dependent deoxyribonucleic acid polymerase in the virions.

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