Abstract
Ten bacteriophages that attack and lyse saprophytic strains of Bdellovibrio bacteriovorus were isolated. Morphological, serological, and host-range studies revealed that there were four different bdellovibrio phages present among the isolates. One of the phages lysed a strain of B. bacteriovorus that requires the presence of a suitable bacterial host for growth. The phage attached to the bdellovibrio cells in the absence of the bacterial host cells; lysis occurred only in the presence of host cells. The 19 saprophytic bdellovibrio strains employed in the phage host-range studies were grouped on the basis of their susceptibility to phage lysis.
MeSH Terms
Antigens, Viral
Bacteria/classification,growth & development
Bacteriophages/growth & development,immunology,isolation & purification
Centrifugation, Density Gradient
Cross Reactions
Escherichia coli
Lysogeny
Microscopy, Electron
Neutralization Tests
Staining and Labeling
Sucrose
Viral Plaque Assay
Viral Proteins
Chemicals
Antigens, Viral
Viral Proteins
Sucrose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Althauser M
Samsonoff W A
Anderson C
Conti S F
References (15)
15 references, click to expand
-
Lysis of Gram-negative bacteria by host-independent ectoparasitic Bdellovibrio bacteriovorus isolates.
J Gen Microbiol. 1965 Sep;40(3):317-28
PMID: 5325037
-
The fluorescent staining of bacteriophage nucleic acids.
J Gen Microbiol. 1966 Sep;44(3):383-91
PMID: 5971386
-
Ultrastructure of bacteriophage and bacteriocins.
Bacteriol Rev. 1967 Dec;31(4):230-314
PMID: 4865539
-
Electron microscopic observations on the penetration of Bdellovibrio bacteriovorus into gram-negative bacterial hosts.
J Bacteriol. 1968 Oct;96(4):1366-81
PMID: 4879563
-
[Bdellovibrio bacteriovorus--a predatory bacterial parasite].
Naturwissenschaften. 1968 Feb;55(2):57-63
PMID: 4883361
-
Factors affecting the intracellular parasitic growth of Bdellovibrio bacteriovorus developing within Escherichia coli.
J Bacteriol. 1969 Feb;97(2):912-23
PMID: 4886299
-
Isolation and characterization of host-independent Bdellovibrios.
J Bacteriol. 1969 Nov;100(2):769-85
PMID: 4901359
-
Deoxyribonucleic acid characterization of Bdellovibrios.
J Bacteriol. 1969 Nov;100(2):786-90
PMID: 4901360
-
Facultatively parasitic strain of Bdellovibrio bacteriovorus.
J Bacteriol. 1970 Mar;101(3):989-96
PMID: 4908792
-
Isolation of a bacteriophage for Bdellovibrio bacteriovorus.
J Virol. 1970 Jan;5(1):97-8
PMID: 4191817
-
Molecular heterogeneity of the Bdellovibrios: evidence of two new species.
J Bacteriol. 1972 Jan;109(1):209-17
PMID: 4621626
-
Three-membered parasitic system: a bacteriophage, Bdellovibrio bacteriovorus, and Escherichia coli.
J Virol. 1972 Mar;9(3):519-25
PMID: 4552554
-
The Bdellovibros.
Annu Rev Microbiol. 1971;25:649-78
PMID: 4949041
-
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75
PMID: 14907713
-
BDELLOVIBRIO BACTERIOVORUS GEN. ET SP. N., A PREDATORY, ECTOPARASITIC, AND BACTERIOLYTIC MICROORGANISM.
Antonie Van Leeuwenhoek. 1963;29:217-48
PMID: 14068454