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PMID: 4114321 Published · ppublish English Journal Article

Nuclear disruption after infection of Escherichia coli with a bacteriophage T4 mutant unable to induce endonuclease II.

Journal of virology ·Vol. 10 ·No. 1 ·1972-07-00 ·Pages 124-33

Snustad DP, Warner HR, Parson KA, Anderson DL

Abstract

Nuclear disruption after infection of Escherichia coli with a bacteriophage T4 mutant deficient in the ability to induce endonuclease II indicates that either (i) the endonuclease II-catalyzed reaction is not the first step in host deoxyribonucleic acid (DNA) breakdown or (ii) nuclear disruption is independent of nucleolytic cleavage of the host chromosome. M-band analysis demonstrates that the host DNA remains membrane-bound after infection with either an endonuclease II-deficient mutant or T4 phage ghosts.

MeSH Terms
Carbon Isotopes Cell Nucleus Centrifugation, Density Gradient Chromosomes, Bacterial Coliphages/enzymology,growth & development,metabolism DNA, Bacterial/metabolism DNA, Viral/biosynthesis Defective Viruses Deoxyribonucleases/biosynthesis Enzyme Induction Escherichia coli/cytology,metabolism Genes Genetics, Microbial Inclusion Bodies, Viral Microscopy, Electron Mutation Staining and Labeling Sucrose Thymidine/metabolism Viral Proteins/pharmacology
Chemicals
Carbon Isotopes DNA, Bacterial DNA, Viral Viral Proteins Sucrose Deoxyribonucleases Thymidine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Snustad D P
Warner H R
Parson K A
Anderson D L
References (23)
23 references, click to expand
  1. Thymidine and thymine incorporation into deoxyribonucleic acid: inhibition and repression by uridine of thymidine phosphorylase of Escherichia coli.
    J Bacteriol. 1967 Nov;94(5):1546-50 PMID: 4862197
  2. DNA replication studied by a new method for the isolation of cell membrane-DNA complexes.
    Cold Spring Harb Symp Quant Biol. 1968;33:707-10 PMID: 4977759
  3. Bacteriophage-induced inhibition of host functions. II. Evidence for multiple, sequential bacteriophage-induced deoxyribonucleases responsible for degradation of cellular deoxyribonucleic acid.
    J Virol. 1969 Jun;3(6):549-56 PMID: 4894764
  4. Enzymatic breakage of deoxyribonucleic acid. I. Purification and properties of endonuclease II from T4 phage-infected Escherichia coli.
    J Biol Chem. 1969 Nov 25;244(22):6182-91 PMID: 4310836
  5. Isolation of bacteriophage T4 mutants defective in the ability to degrade host deoxyribonucleic acid.
    J Virol. 1970 Jun;5(6):700-8 PMID: 4914096
  6. Biological activity of bacteriophage ghosts and "take-over" of host functions by bacteriophage.
    Bacteriol Rev. 1970 Sep;34(3):344-63 PMID: 4918524
  7. Enzymes of nucleic acid metabolism.
    Annu Rev Biochem. 1970;39:291-322 PMID: 4920825
  8. Inhibition of T4 bacteriophage multiplication by superinfecting ghosts and the development of tolerance after bacteriophage infection.
    J Virol. 1971 Jan;7(1):8-14 PMID: 5543436
  9. Mutants in a nonessential gene of bacteriophage T4 which are defective in the degradation of Escherichia coli deoxyribonucleic acid.
    J Virol. 1971 Jan;7(1):95-105 PMID: 5543437
  10. Mutants of bacteriophage T4 defective in the induction of T4 endonuclease II.
    J Biol Chem. 1971 May 25;246(10):3431-3 PMID: 4930062
  11. The utilization of host pyrimidines in the synthesis of bacterial viruses.
    J Biol Chem. 1951 Oct;192(2):693-700 PMID: 14907663
  12. Biochemical studies of virus reproduction. VIII. Purine metabolism.
    J Biol Chem. 1952 May;197(1):113-20 PMID: 12981040
  13. Nucleic acid economy in bacteria infected with bacteriophage T2. I. Purine and pyrimidine composition.
    J Gen Physiol. 1953 Jul;36(6):777-89 PMID: 13069681
  14. Origin and fate of bacteriophage material.
    Cold Spring Harb Symp Quant Biol. 1953;18:209-20 PMID: 13168988
  15. [Study of the action of membrane preparations of bacteriophage T2 on Escherichia coli].
    Biochim Biophys Acta. 1955 Mar;16(3):330-8 PMID: 14378159
  16. The protein coats or ghosts of coliphage T2. I. Preparation, assay, and some chemical properties.
    J Gen Physiol. 1957 May 20;40(5):809-25 PMID: 13428990
  17. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states.
    J Biophys Biochem Cytol. 1958 Nov 25;4(6):671-8 PMID: 13610928
  18. Improvements in epoxy resin embedding methods.
    J Biophys Biochem Cytol. 1961 Feb;9:409-14 PMID: 13764136
  19. A critical test of a current theory of genetic recombination in bacteriophage.
    Genetics. 1962 Feb;47:187-208 PMID: 13916671
  20. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
    J Cell Biol. 1963 Apr;17:208-12 PMID: 13986422
  21. Chromatin staining of bacteria during bacteriophage infection.
    J Bacteriol. 1950 Apr;59(4):551-60 PMID: 15436430
  22. Cytological changes in Escherichia coli produced by infection with phage T2.
    J Bacteriol. 1950 May;59(5):603-15 PMID: 15436434
  23. Electron microscopical studies of phage multiplication. IV. The establishment of the DNA pool of vegetative phage and the maturation of phage particles.
    Virology. 1959 Aug;8:478-98 PMID: 14405215
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.10.1.124-133.1972
Published
1972-07-00
Pages
124-33
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356433
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.10.1.124-133.1972 PMC Full Text View journal details
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