Studies on the adsorption of certain medium proteins to Mycoplasma gallisepticum and their influence on agglutination and haemagglutination reactions.

The Journal of hygiene ·Vol. 70 ·No. 2 ·1972-06-00 ·Pages 267-78

Bradbury JM, Jordan FT

Abstract

Serum proteins from Mycoplasma gallisepticum culture medium could be detected on the organisms as a result of incubation at low pH. Only certain of the serum proteins, including IgG and IgM, were found, and the adsorption appears to depress the haemagglutinating activity of the organisms. There was no obvious effect on slide agglutination (SA) sensitivity but an incidental finding was that brief acid treatment enhanced the SA sensitivity of an antigen prepared from a young culture.

MeSH Terms

Adsorption Agglutination/drug effects Animals Antigens, Bacterial Blood Proteins/metabolism,pharmacology Chickens Culture Media Hemagglutination/drug effects Hydrogen-Ion Concentration Immune Sera Immunoelectrophoresis Immunoglobulin G/analysis Immunoglobulins/analysis Mycoplasma/immunology Rabbits Swine Transferrin/analysis Turkeys gamma-Globulins/analysis

Chemicals

Antigens, Bacterial Blood Proteins Culture Media Immune Sera Immunoglobulin G Immunoglobulins Transferrin gamma-Globulins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Bradbury J M

Jordan F T

References (11)

11 references, click to expand

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Immunoelectrophoresis of normal pig serum.
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A general study on the adsorption of protein by tissue.
Biochim Biophys Acta. 1968 Feb 19;154(2):269-77 PMID: 4170138
Proteins adhering to Escherichia coli after exposure to guinea-pig serum.
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Non-specific antibodies in chickens inoculated intratracheally with Mycoplasma gallisepticum.
J Comp Pathol. 1968 Oct;78(4):407-14 PMID: 5695834
The absorption of -globulins to Mycoplasma gallisepticum and the possible role in non-specific serological reactions.
Vet Rec. 1971 Sep 11;89(11):318 PMID: 4106502
The influence of pH of the culture medium on the sensitivity of Mycoplasma gallisepticum antigens for use in certain serological tests.
J Hyg (Lond). 1971 Dec;69(4):593-606 PMID: 5002646
ANTIGENIC SPECIFICITIES ACQUIRED FROM THE GROWTH MEDIUM BY CELLS IN TISSUE CULTURE.
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[A micro-method of immuno-electrophoresis].
Int Arch Allergy Appl Immunol. 1955;7(2):103-10 PMID: 13262900
Products of glucose metabolism by pleuropneumonialike organisms.
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Ann N Y Acad Sci. 1960 Jan 15;79:521-30 PMID: 13839052

Article Info

Journal

The Journal of hygiene

Abbr.

J Hyg (Lond)

ISSN

0022-1724

DOI

10.1017/s0022172400022324

Published

1972-06-00

Pages

267-78

Language

English

Region

England

NLM ID

0375374

PMCID

PMC2130043

Subset

External Links

PubMed source DOI: 10.1017/s0022172400022324 PMC Full Text View journal details

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Studies on the adsorption of certain medium proteins to Mycoplasma gallisepticum and their influence on agglutination and haemagglutination reactions.

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