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PMID: 4110107 Published · ppublish English Journal Article

Reduction and reoxidation of Australia antigen: loss and reconstitution of particle structure and antigenicity.

Journal of virology ·Vol. 9 ·No. 1 ·1972-01-00 ·Pages 182-3

Sukeno N, Shirachi R, Yamaguchi J, Ishida N

Abstract

Reduction of Australia antigen with dithiothreitol resulted in the loss of antigenicity and morphological integrity. Oxidation of the reduced Australia antigen reconstituted both of these characters.

MeSH Terms
Acetamides Alkylating Agents Alkylation Centrifugation, Density Gradient Cesium Chlorides Chromatography, DEAE-Cellulose Dialysis Dithiothreitol Hepatitis B Antigens/isolation & purification Hepatitis B virus/immunology Iodoacetates Microscopy, Electron Oxidation-Reduction Phosphotungstic Acid Staining and Labeling Sucrose
Chemicals
Acetamides Alkylating Agents Chlorides Hepatitis B Antigens Iodoacetates Phosphotungstic Acid Cesium Sucrose Dithiothreitol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sukeno N
Shirachi R
Yamaguchi J
Ishida N
References (8)
8 references, click to expand
  1. Biophysical properties of Australia antigen.
    J Virol. 1969 Nov;4(5):763-8 PMID: 5360526
  2. Some antigenic and physical properties of virus-like particles in sera of hepatitis patients.
    J Immunol. 1969 Jun;102(6):1529-32 PMID: 5785352
  3. DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPS.
    Biochemistry. 1964 Apr;3:480-2 PMID: 14192894
  4. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  5. Australia antigen: large-scale purification from human serum and biochemical studies of its proteins.
    J Virol. 1971 May;7(5):569-76 PMID: 5105002
  6. Serum hepatitis antigen (SH): rapid detection by high voltage immunoelectroosmophoresis.
    Science. 1970 Aug 7;169(3945):593-5 PMID: 4987681
  7. Australia antigen (a hepatitis-associated antigen): purification and physical properties.
    J Exp Med. 1970 Jun 1;131(6):1190-9 PMID: 4246140
  8. Rapid detection of Australia antigen by counterimmunoelectrophoresis.
    J Immunol. 1970 Apr;104(4):1031-4 PMID: 4986160
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.9.1.182-183.1972
Published
1972-01-00
Pages
182-3
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356275
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.9.1.182-183.1972 PMC Full Text View journal details
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