Use of antiviral-antiferritin hybrid antibody for localization of viral antigen in plasma membrane.

Journal of virology ·Vol. 7 ·No. 2 ·1971-02-00 ·Pages 274-7

Wagner RR, Heine JW, Goldstein G, Schnaitman CA

Abstract

Reduced Fab' fragments of viral antibody hybridized with reduced Fab' fragments of antiferritin immunoglobulin G bind to viral antigenic sites in the plasma membrane of L cells infected with vesicular stomatitis virus. The hybrid antibody reacts specifically with ferritin, which can be identified by electron microscopy, and with fluorescein-conjugated apoferritin, which can be identified by fluorescence microscopy.

MeSH Terms

Animals Antibodies Antigens/isolation & purification Cell Membrane/immunology Ferritins Fluorescent Antibody Technique Immune Sera Immunochemistry Immunoglobulin G L Cells/immunology Methods Microscopy, Electron Microscopy, Fluorescence Rabbits Staining and Labeling Time Factors Vesicular stomatitis Indiana virus/immunology,pathogenicity

Chemicals

Antibodies Antigens Immune Sera Immunoglobulin G Ferritins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wagner R R

Heine J W

Goldstein G

Schnaitman C A

References (6)

6 references, click to expand

Use of hybrid antibody with anti-gamma-G and anti-ferritin specificities in locating cell surface antigens by electron microscopy.
J Exp Med. 1968 Dec 1;128(6):1461-73 PMID: 4972125
Fusion of vesicular stomatitis virus with the cytoplasmic membrane of L cells.
J Virol. 1969 Jun;3(6):619-22 PMID: 4307905
Concentration and purification of vesicular stomatitis virus by polyethylene glycol "precipitation".
Virology. 1970 Mar;40(3):745-6 PMID: 4314510
Lipid composition of purified vesicular stomatitis viruses.
J Virol. 1971 Jan;7(1):59-70 PMID: 5101092
Recombination of a mixture of univalent antibody fragments of different specificity.
Arch Biochem Biophys. 1961 May;93:460-2 PMID: 13729244
BIOLOGIC PROPERTIES OF TWO PLAQUE VARIANTS OF VESICULAR STOMATITIS VIRUS (INDIANA SEROTYPE).
J Immunol. 1963 Jul;91:112-22 PMID: 14056363

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.7.2.274-277.1971

Published

1971-02-00

Pages

274-7

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356109

Subset

External Links

PubMed source DOI: 10.1128/JVI.7.2.274-277.1971 PMC Full Text View journal details

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Use of antiviral-antiferritin hybrid antibody for localization of viral antigen in plasma membrane.

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