Isolation of bacteriophages T2 and T4 attached to the outer membrane of Escherichia coli.

Journal of virology ·Vol. 7 ·No. 5 ·1971-05-00 ·Pages 683-6

DePamphilis ML

Abstract

Phage T2 or T4 was adsorbed to Escherichia coli, and the outer (L) membrane was then isolated with the phage still attached in their usual postinjection appearance. T2 was readily inactivated by isolated cell walls but very poorly by purified L membrane. T4 was inactivated by neither.

MeSH Terms

Adsorption Buffers Cell Wall Centrifugation, Density Gradient Cesium Chlorides Coliphages/isolation & purification Escherichia coli Lipopolysaccharides/isolation & purification Magnesium Methods Microscopy, Electron Phosphotungstic Acid Staining and Labeling Sucrose Tromethamine

Chemicals

Buffers Chlorides Lipopolysaccharides Tromethamine Phosphotungstic Acid Cesium Sucrose Magnesium

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

DePamphilis M L

References (17)

17 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.7.5.683-686.1971

Published

1971-05-00

Pages

683-6

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356180

Subset

External Links

PubMed source DOI: 10.1128/JVI.7.5.683-686.1971 PMC Full Text View journal details

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Isolation of bacteriophages T2 and T4 attached to the outer membrane of Escherichia coli.

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