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PMID: 4085302 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA hypomethylation causes an increase in DNase-I sensitivity and an advance in the time of replication of the entire inactive X chromosome.

Chromosoma ·Vol. 93 ·No. 2 ·1985-00-00 ·Pages 152-6

Jablonka E, Goitein R, Marcus M, Cedar H

Abstract

We have examined the effect of 5-azacytidine (5-aza-C) induced hypomethylation of DNA on the time of replication and DNase I sensitivity of the X chromosomes of female Gerbillus gerbillus (rodent) lung fibroblast cells. Using in situ nick translation to visualise the potential state of activity of large regions of metaphase chromosomes we show that 5-aza-C causes a dramatic increase in the DNase-I sensitivity of the entire inactive X chromosome of female G. gerbillus cells and this increase in nuclease sensitivity correlates with a large shift in the time of replication of the inactive X chromosome from late S phase to early S phase. These effects of 5-aza-C on the inactive X chromosome are associated with a 15% decrease in DNA methylation. Our results indicate that DNA methylation concomitantly affects both the time of replication and the chromatin conformation of the inactive X chromosome.

MeSH Terms
Animals Cells, Cultured DNA/genetics DNA Replication Deoxyribonuclease I/metabolism Female Gerbillinae Karyotyping Kinetics Lung/cytology Methylation X Chromosome
Chemicals
DNA Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jablonka E
Goitein R
Marcus M
Cedar H
References (20)
20 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00293162
Published
1985-00-00
Pages
152-6
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Grants
NIGMS NIH HHS · GM32745-02 · United States
External Links
PubMed source DOI: 10.1007/BF00293162 View journal details
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