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PMID: 4074745 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel approach to the isolation of rabbit reticulocyte haem-controlled eIF-2 alpha protein kinase.

Biochimica et biophysica acta ·Vol. 826 ·No. 4 ·1985-12-18 ·Pages 224-8

Jackson RJ, Hunt T

Abstract

A new strategy for the purification of rabbit reticulocyte haem-controlled eIF-2 alpha kinase is described, based on the fact that this kinase can be self-phosphorylated in several sites. Incubation of partially purified kinase with ATP changes its behaviour on anion exchangers sufficiently to separate it from almost all contaminating proteins.

MeSH Terms
Animals Chromatography, Ion Exchange Heme/pharmacology Kinetics Phosphorylation Protein Kinases/blood,isolation & purification Rabbits Reticulocytes/enzymology eIF-2 Kinase
Chemicals
Heme Protein Kinases eIF-2 Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jackson R J
Hunt T
Article Info
Journal
Biochimica et biophysica acta
Abbr.
Biochim Biophys Acta
ISSN
0006-3002
DOI
10.1016/0167-4781(85)90010-7
Published
1985-12-18
Pages
224-8
Language
English
Region
Netherlands
NLM ID
0217513
Subset
IM
External Links
PubMed source DOI: 10.1016/0167-4781(85)90010-7
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