Home LiteratureArticle Details
PMID: 406831 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Inducible resistance to D-cycloserine in Bacillus subtilis 168.

Antimicrobial agents and chemotherapy ·Vol. 11 ·No. 5 ·1977-05-00 ·Pages 871-6

Clark VL, Young FE

Abstract

Resistance to d-cycloserine could be induced in Bacillus subtilis 168 by sublethal concentrations of d-cycloserine. Sensitivity to the antibiotic could be regained by growth in the absence of d-cycloserine. The bactericidal activity of d-cycloserine apparently was not altered by resistant cells, and peptidoglycan synthesis was still inhibited by d-cycloserine in resistant cells. The d-cycloserine resistance apparently resulted from a decreased uptake of the antibiotic. The decrease in d-cycloserine transport could be prevented by simultaneous treatment of the cells with rifampin and d-cycloserine. d-Cycloserine was transported by the same system as glycine in B. subtilis. d-Cycloserine was able to exchange for intracellular glycine in both sensitive and resistant cells, suggesting that d-cycloserine is not excluded from the cell in resistant cultures.

MeSH Terms
Bacillus subtilis/drug effects,growth & development,metabolism Biological Transport, Active/drug effects Cycloserine/metabolism,pharmacology Drug Resistance, Microbial Glycine/metabolism Peptidoglycan/metabolism Time Factors
Chemicals
Peptidoglycan Cycloserine Glycine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clark V L
Young F E
References (14)
14 references, click to expand
  1. Isolation and characterization of tetracycline resistance proteins from Staphylococcus aureus and Escherichia coli.
    Antimicrob Agents Chemother. 1976 Mar;9(3):526-34 PMID: 769681
  2. R-factor-mediated resistance to tetracycline in Escherichia coli K12: an R-factor with a mutation to temperature-sensitive tetracycline resistance.
    Biochem Biophys Res Commun. 1976 May 3;70(1):117-24 PMID: 776184
  3. Synthesis of an R plasmid protein associated with tetracycline resistance is negatively regulated.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1509-12 PMID: 775491
  4. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.
    Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 PMID: 16590310
  5. A fractionation procedure for studies of the synthesis of cell-wall mucopeptide and of other polymers in cells of Staphylococcus aureus.
    J Gen Microbiol. 1960 Feb;22:249-58 PMID: 14430471
  6. THE ENZYMATIC SYNTHESIS OF D-ALANYL-D-ALANINE. 3. ON THE INHIBITION OF D-ALANYL-D-ALANINE SYNTHETASE BY THE ANTIBIOTIC D-CYCLOSERINE.
    Biochemistry. 1964 Apr;3:471-80 PMID: 14188160
  7. Enzymes that inactivate antibiotics in transit to their targets.
    Ann N Y Acad Sci. 1974 May 10;235(0):130-6 PMID: 4527785
  8. Mechanism of D-cycloserine action: alanine racemase from Escherichia coli W.
    J Bacteriol. 1972 Jun;110(3):978-87 PMID: 4555420
  9. Mechanism of D-cycloserine action: transport systems for D-alanine, D-cycloserine, L-alanine, and glycine.
    J Bacteriol. 1970 Sep;103(3):778-88 PMID: 4919992
  10. Mechanism of D-cycloserine action: transport mutants for D-alanine, D-cycloserine, and glycine.
    J Bacteriol. 1971 Mar;105(3):1028-35 PMID: 4926674
  11. Active transport of D-alanine and related amino acids by whole cells of Bacillus subtilis.
    J Bacteriol. 1974 Dec;120(3):1085-92 PMID: 4215790
  12. Genetics of amino acid transport in bacteria.
    Annu Rev Genet. 1974;8:103-33 PMID: 4613254
  13. Mechanisms of antibiotic resistance in bacteria.
    Annu Rev Biochem. 1973;42:471-506 PMID: 4581231
  14. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.11.5.871
Published
1977-05-00
Pages
871-6
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC352089
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.11.5.871 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2