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PMID: 4066748 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Synchronous exocytosis in Paramecium cells involves very rapid (less than or equal to 1 s), reversible dephosphorylation of a 65-kD phosphoprotein in exocytosis-competent strains.

The Journal of cell biology ·Vol. 101 ·No. 6 ·1985-12-00 ·Pages 2028-35

Zieseniss E, Plattner H

Abstract

Synchronous exocytosis in Paramecium cells involves the rapid (less than or equal to 1 s) dephosphorylation of a 65-kD phosphoprotein, which, after a lag phase of approximately 5 s, is reversed within approximately 20 s. Exocytosis inhibitors suppress this reaction; stimulatory and inhibitory effects are dose dependent. The dephosphorylation of the 65-kD phosphoprotein occurs only in exocytosis-competent strains, but not in mutant strains that cannot carry out membrane fusion, or that are devoid of secretory organelles or cannot transport them to the cell membrane. Since under all conditions analyzed the transient dephosphorylation of the 65-kD phosphoprotein strictly parallels the actual amount of exocytosed organelles, this process might be involved in exocytosis performance, perhaps in its initiation.

MeSH Terms
Animals Exocytosis Kinetics Molecular Weight Mutation Neomycin/pharmacology Paramecium/physiology Phosphoproteins/physiology Phosphorylation Temperature
Chemicals
Phosphoproteins Neomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zieseniss E
Plattner H
References (38)
38 references, click to expand
  1. Calcium-regulated phosphorylation in synaptosomal cytosol: dependence on calmodulin.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5812-6 PMID: 6934513
  2. Control of membrane fusion in exocytosis. Physiological studies on a Paramecium mutant blocked in the final step of the trichocyst extrusion process.
    J Cell Biol. 1980 May;85(2):213-27 PMID: 7372705
  3. Secretory protein decondensation as a distinct, Ca2+-mediated event during the final steps of exocytosis in Paramecium cells.
    J Cell Biol. 1981 Jan;88(1):179-88 PMID: 7204486
  4. Control of exocytotic processes: cytological and physiological studies of trichocyst mutants in Paramecium tetraurelia.
    J Cell Biol. 1981 Feb;88(2):301-11 PMID: 7204496
  5. Phosphorylation of adrenal medulla cell proteins in conjunction with stimulation of catecholamine secretion.
    J Neurochem. 1981 Mar;36(3):847-54 PMID: 7205277
  6. Calcium-dependent protein kinase: widespread occurrence in various tissues and phyla of the animal kingdom and comparison of effects of phospholipid, calmodulin, and trifluoperazine.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7039-43 PMID: 6938952
  7. Genetic dissection of the final exocytosis steps in Paramecium tetraurelia cells: cytochemical determination of Ca2+-ATPase activity over performed exocytosis sites.
    J Cell Sci. 1980 Dec;46:17-40 PMID: 6453127
  8. beta-Adrenergic regulation of protein phosphorylation and its relationship to exocrine secretion in dispersed rat parotid gland acinar cells.
    J Biol Chem. 1981 Sep 25;256(18):9731-6 PMID: 6270099
  9. Effects of Ca2+, calmodulin and cyclic AMP on the phosphorylation of endogenous proteins by homogenates of rt islets of langerhans.
    Biochim Biophys Acta. 1982 Feb 2;714(2):313-9 PMID: 6275912
  10. Cytoskeleton-secretory vesicle interactions during the docking of secretory vesicles at the cell membrane in Paramecium tetraurelia cells.
    J Cell Biol. 1982 Feb;92(2):368-77 PMID: 7199530
  11. Microinjection of cytoplasm as a test of complementation in Paramecium.
    J Cell Biol. 1982 Feb;92(2):559-64 PMID: 7061597
  12. The calmodulin hypothesis of neurotransmission.
    Cell Calcium. 1981 Aug;2(4):365-85 PMID: 6122506
  13. Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini.
    J Biol Chem. 1982 Sep 10;257(17):10523-8 PMID: 7050109
  14. Phosphoproteins of the adrenal chromaffin granule membrane.
    J Neurochem. 1982 Nov;39(5):1387-96 PMID: 6288873
  15. Protein phosphorylation/dephosphorylation and stimulus-secretion coupling in wild type and mutant Paramecium.
    J Biol Chem. 1982 Dec 10;257(23):13903-6 PMID: 7142183
  16. Regulation of polyamine-responsive protein kinase by certain highly specific polyamines and charged carbohydrates.
    Biochim Biophys Acta. 1983 Feb 22;755(3):358-62 PMID: 6824733
  17. The involvement of protein phosphorylation in stimulus-secretion coupling in the mouse exocrine pancreas.
    Biochem J. 1983 Feb 15;210(2):353-9 PMID: 6134521
  18. Ca2+-dependent binding of cytosolic components to insulin-secretory granules results in Ca2+-dependent protein phosphorylation.
    Biochem J. 1983 Feb 15;210(2):533-9 PMID: 6305343
  19. Polyamines inhibit phospholipid-sensitive and calmodulin-sensitive Ca2+-dependent protein kinases.
    Biochem J. 1983 Aug 1;213(2):281-8 PMID: 6615435
  20. Phosphorylation of endogenous proteins of cilia from Paramecium tetraurelia in vitro.
    Eur J Cell Biol. 1983 Sep;31(2):220-6 PMID: 6315438
  21. Synchronous exocytosis in Paramecium cells. III. Rearrangement of membranes and membrane-associated structural elements after exocytosis performance.
    Exp Cell Res. 1984 Mar;151(1):21-8 PMID: 6698118
  22. Synchronous exocytosis in Paramecium cells. I. A novel approach.
    Exp Cell Res. 1984 Mar;151(1):6-13 PMID: 6199221
  23. Phosphorylation of zymogen granule membrane proteins in intact rat pancreatic acinar cells.
    Biochem Biophys Res Commun. 1984 Jul 18;122(1):413-9 PMID: 6743341
  24. Neuronal phosphoproteins: physiological and clinical implications.
    Science. 1984 Sep 21;225(4668):1357-64 PMID: 6474180
  25. Subcellular location of stimulus-affected endogenous phosphoproteins in the rat parotid gland.
    J Cell Biol. 1984 Oct;99(4 Pt 1):1354-63 PMID: 6148346
  26. Regulation of protein phosphorylation by polyamines in hepatocytes.
    Biochim Biophys Acta. 1984 Oct 16;801(3):461-9 PMID: 6487653
  27. Synchronous exocytosis in Paramecium cells. IV. Polyamino compounds as potent trigger agents for repeatable trigger-redocking cycles.
    Eur J Cell Biol. 1985 Jan;36(1):32-7 PMID: 2579814
  28. Synchronous exocytosis in Paramecium cells. V. Ultrastructural adaptation phenomena during re-insertion of secretory organelles.
    Eur J Cell Biol. 1985 Jan;36(1):38-47 PMID: 3979401
  29. Reactivated triton-extracted models o paramecium: modification of ciliary movement by calcium ions.
    Science. 1972 May 5;176(4034):523-4 PMID: 5032354
  30. Membrane specializations in the form of regular membrane-to-membrane attachment sites in Paramecium. A correlated freeze-etching and ultrathin-sectioning analysis.
    J Cell Sci. 1973 Nov;13(3):687-719 PMID: 4772364
  31. Mutations affecting the trichocysts in Paramecium aurelia. I. Morphology and description of the mutants.
    J Protozool. 1974 May;21(2):352-62 PMID: 4599165
  32. Genetic analysis of membrane differentiation in Paramecium. Freeze-fracture study of the trichocyst cycle in wild-type and mutant strains.
    J Cell Biol. 1976 Apr;69(1):126-43 PMID: 1254639
  33. Calcium-dependent protein phosphorylation during secretion by exocytosis in the mast cell.
    Nature. 1978 Sep 28;275(5678):329-31 PMID: 357989
  34. Ca2+-dependent protein phosphorylation system in membranes from various tissues, and its activation by "calcium-dependent regulator".
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5432-6 PMID: 214787
  35. Phosphorylation-dephosphorylation of enzymes.
    Annu Rev Biochem. 1979;48:923-59 PMID: 38740
  36. Antiallergic drug cromolyn may inhibit histamine secretion by regulating phosphorylation of a mast cell protein.
    Science. 1980 Jan 4;207(4426):80-2 PMID: 6153130
  37. The role of calmodulin in the regulation of protein phosphorylation and insulin release in hamster insulinoma cells.
    J Biol Chem. 1980 May 10;255(9):4120-4 PMID: 6246114
  38. Genetic analysis of the relationships between the cell surface and the nuclei in Paramecium tetraurella.
    Genetics. 1980 Aug;95(4):797-818 PMID: 7203007
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.101.6.2028
Published
1985-12-00
Pages
2028-35
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114016
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.101.6.2028 PMC Full Text View journal details
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