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PMID: 405370 Published · ppublish English Journal Article

Biosynthesis of wall polymers in Bacillus subtilis.

Journal of bacteriology ·Vol. 130 ·No. 3 ·1977-06-00 ·Pages 1055-63

Wyke AW, Ward JB

Abstract

Preparations of membrane plus wall derived from Bacillus subtilis W23 were used to study the in vitro synthesis of peptidoglycan and teichoic acid and their linkage to the preexisting cell wall. The teichoic acid synthesis showed an ordered requirement for the incorporation of N-acetylglucosamine from uridine 5'-diphosphate (UDP)-N-acetylglucosamine followed by addition of glycerol phosphate from cytidine 5'-diphosphate (CDP)-glycerol and finally by addition of ribitol phosphate from CDP-ribitol. UDP-N-acetylglucosamine was not only required for the synthesis of the teichoic acid, but N-acetylglucosamine residues formed an integral part of the linkage unit attaching polyribitol phosphate to the cell wall. Synthesis of the teichoic acid was exquisitely sensitive to the antibiotic tunicamycin, and this was shown to be due to the inhibition of incorporation of N-acetylglucosamine units from UDP-N-acetylglucosamine.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacillus subtilis/drug effects,metabolism,ultrastructure Cell Membrane/drug effects,metabolism Cell Wall/drug effects,metabolism Chemical Phenomena Chemistry Glucosamine/analogs & derivatives,pharmacology Lipid Metabolism Peptidoglycan/biosynthesis Teichoic Acids/biosynthesis,metabolism Uridine Diphosphate N-Acetylglucosamine/metabolism
Chemicals
Anti-Bacterial Agents Peptidoglycan Teichoic Acids Uridine Diphosphate N-Acetylglucosamine Glucosamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wyke A W
Ward J B
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29 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.130.3.1055-1063.1977
Published
1977-06-00
Pages
1055-63
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235327
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.130.3.1055-1063.1977 PMC Full Text View journal details
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