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PMID: 4030100 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of Staphylococcus aureus clumping factor.

Infection and immunity ·Vol. 49 ·No. 3 ·1985-09-00 ·Pages 700-8

Espersen F, Clemmensen I, Barkholt V

Abstract

Immunochemically identical components were isolated from water-soluble phases of five Staphylococcus aureus strains by affinity chromatography on fibrinogen-linked Sepharose 4B. The elution was performed with 1 M MgCl2. The component could be isolated from sonicated preparations of whole cells, cell walls, and extracellular products of S. aureus but not from sonicated preparations of staphylococcal L-forms or from Staphylococcus epidermidis. Investigations of the eluted component by immunoelectrophoresis and Western blot analysis by use of different polyspecific antibodies to S. aureus raised in rabbits revealed only one immunoprecipitate or one band. By means of gel filtration on Sepharose CL 6B and sodium dodecyl sulfate-polyacrylamide gel electrophoresis a molecular mass of 420,000 and 360,000 was found, respectively. Chemical analysis showed a carbohydrate content of about 20% by weight. By crossed immunoelectrophoresis the isolated component was demonstrated to bind to human fibrinogen. The finding that this purified component inhibited the fibrinogen-induced clumping of staphylococci strongly suggests that the component is the S. aureus clumping factor.

MeSH Terms
Animals Chromatography, Affinity Chromatography, Gel Coagulase/analysis,isolation & purification Electrophoresis, Polyacrylamide Gel Fibrinogen/pharmacology Immunoelectrophoresis, Two-Dimensional Molecular Weight Rabbits Staphylococcus aureus/immunology
Chemicals
Coagulase Fibrinogen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Espersen F
Clemmensen I
Barkholt V
References (36)
36 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.49.3.700-708.1985
Published
1985-09-00
Pages
700-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC261249
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.49.3.700-708.1985 PMC Full Text View journal details
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