Home LiteratureArticle Details

PMID: 4014674 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Proteins transferred to nitrocellulose for use as immunogens.

Analytical biochemistry ·Vol. 147 ·No. 2 ·1985-06-00 ·Pages 285-8

Knudsen KA

Abstract

Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and were eluted efficiently from the gel by transfer to nitrocellulose paper. The protein-bearing nitrocellulose was solubilized with dimethyl sulfoxide and used as an immunogen in rabbits. Polyclonal antibodies to the proteins were generated.

MeSH Terms

Animals Antibodies, Monoclonal/immunology Chick Embryo Chromatography, Affinity Collodion Dimethyl Sulfoxide/pharmacology Electrophoresis, Polyacrylamide Gel Immunoassay/methods Proteins/immunology,isolation & purification Rabbits

Chemicals

Antibodies, Monoclonal Proteins Collodion Dimethyl Sulfoxide

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Knudsen K A

Article Info

Journal

Analytical biochemistry

Abbr.

Anal Biochem

ISSN

0003-2697

DOI

10.1016/0003-2697(85)90273-8

Published

1985-06-00

Pages

285-8

Language

English

Region

United States

NLM ID

0370535

Subset

Grants

NICHD NIH HHS · HD/CA-15663 · United States

External Links

PubMed source DOI: 10.1016/0003-2697(85)90273-8 View journal details

Analysis Services

Proteins transferred to nitrocellulose for use as immunogens.

Contact

WeChat Official Account