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PMID: 3976840 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Reduced sialylation of podocalyxin--the major sialoprotein of the rat kidney glomerulus--in aminonucleoside nephrosis.

The American journal of pathology ·Vol. 118 ·No. 3 ·1985-03-00 ·Pages 343-9

Kerjaschki D, Vernillo AT, Farquhar MG

Abstract

In this study the sugar composition of podocalyxin was determined in puromycin aminonucleoside-treated (PAN) rats and controls. Podocalyxin from both control and PAN rats bound 125I-WGA and 125I-peanut lectin (the latter only after neuraminidase treatment) on nitrocellulose transfers. Purified podocalyxin from both control and PAN rats was found to contain sialic acid, Gal, GlcNac, and Man but lacked Fuc and GalNac by gas-liquid chromatography. In PAN rats the sialic acid content of podocalyxin was reduced from 4.5% to 1.5%, whereas the concentration of the other sugars (with the possible exception of Gal) was similar to that of controls. The density of podocalyxin on the epithelial cell surface was estimated after immunogold labeling with anti-podocalyxin IgG, and no differences were found between PAN rats and controls. These data indicate that the reduced total glomerular sialic acid content found in PAN is due to the combined effects of the decreased podocyte plasmalemmal surface area and the reduced sialic acid content of podocalyxin.

MeSH Terms
Animals Chromatography, Gas Glycoproteins Kidney Glomerulus/analysis Lectins/metabolism Male Nephrosis/metabolism Polysaccharides/analysis Puromycin Aminonucleoside Rats Rats, Inbred Strains Sialic Acids/analysis Sialoglycoproteins
Chemicals
Glycoproteins Lectins Polysaccharides Sialic Acids Sialoglycoproteins podocalyxin Puromycin Aminonucleoside
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kerjaschki D
Vernillo A T
Farquhar M G
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31 references, click to expand
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1985-03-00
Pages
343-9
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1887944
Subset
IM
Grants
NIADDK NIH HHS · AM 17724 · United States
External Links
PubMed source PMC Full Text View journal details
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